Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27930 | 3' | -57.6 | NC_005886.2 | + | 16677 | 0.66 | 0.603007 |
Target: 5'- cGCAcCGUUCGGcacugcGACGuacgcCGGCAGGUucGGa -3' miRNA: 3'- -CGUaGCAAGCC------CUGCc----GCCGUCCA--CC- -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 36192 | 0.68 | 0.447386 |
Target: 5'- gGCAUCGgcggCGGcacgggcggcaucGGCGGCGGCAcgGGcGGc -3' miRNA: 3'- -CGUAGCaa--GCC-------------CUGCCGCCGU--CCaCC- -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 6797 | 0.69 | 0.401692 |
Target: 5'- uGCAUCaaaCGGG-CGGCGGaaCAGGUGc -3' miRNA: 3'- -CGUAGcaaGCCCuGCCGCC--GUCCACc -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 36243 | 0.71 | 0.303138 |
Target: 5'- gGCAUCGgcggCGgcacGGGCGGCGGCGGcGgcacGGg -3' miRNA: 3'- -CGUAGCaa--GC----CCUGCCGCCGUC-Ca---CC- -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 36312 | 0.72 | 0.261292 |
Target: 5'- gGCAUCGgcggcaUCGGcGGCacgGGCGGCAcgggcGGUGGu -3' miRNA: 3'- -CGUAGCa-----AGCC-CUG---CCGCCGU-----CCACC- -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 36360 | 0.75 | 0.16353 |
Target: 5'- gGCAUCGgcggCGgcacGGGCGGCGGCAGGc-- -3' miRNA: 3'- -CGUAGCaa--GC----CCUGCCGCCGUCCacc -5' |
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27930 | 3' | -57.6 | NC_005886.2 | + | 17727 | 1.11 | 0.000399 |
Target: 5'- cGCAUCGUUCGGGACGGCGGCAGGUGGc -3' miRNA: 3'- -CGUAGCAAGCCCUGCCGCCGUCCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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