Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27932 | 5' | -53.1 | NC_005886.2 | + | 29498 | 0.66 | 0.777324 |
Target: 5'- cAACgACGgUAgGCGCAGCGU-UGACGg -3' miRNA: 3'- -UUG-UGCaAUgCGCGUCGCAgACUGUg -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 37372 | 0.66 | 0.76684 |
Target: 5'- cGACGCGUg--GCGCGGCGcaauacuucgUCgucGGCACg -3' miRNA: 3'- -UUGUGCAaugCGCGUCGC----------AGa--CUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 32989 | 0.66 | 0.765783 |
Target: 5'- cGCGCGUUGCGCGgGaagaccuGCGaagaUGGCACc -3' miRNA: 3'- uUGUGCAAUGCGCgU-------CGCag--ACUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 24767 | 0.66 | 0.756205 |
Target: 5'- -cCGCGUUGCGCGCGGaCG-CggGGCu- -3' miRNA: 3'- uuGUGCAAUGCGCGUC-GCaGa-CUGug -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 10113 | 0.66 | 0.734537 |
Target: 5'- uGAUACGgUGCGCGCAGUaguucGUUUGAUu- -3' miRNA: 3'- -UUGUGCaAUGCGCGUCG-----CAGACUGug -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 6470 | 0.67 | 0.72353 |
Target: 5'- cAGCGCGUUGCGCucGCAuCGcagUGACGCa -3' miRNA: 3'- -UUGUGCAAUGCG--CGUcGCag-ACUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 42918 | 0.67 | 0.678646 |
Target: 5'- cGCACGUUcgAUGCGCAacaauuccGUGUC-GGCGCg -3' miRNA: 3'- uUGUGCAA--UGCGCGU--------CGCAGaCUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 42744 | 0.67 | 0.678646 |
Target: 5'- cAACACGgUAUGCGCuGCGUugCUGAgaACg -3' miRNA: 3'- -UUGUGCaAUGCGCGuCGCA--GACUg-UG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 31938 | 0.67 | 0.667274 |
Target: 5'- cGCGCGUUGCgauccggugcgaGCGCAGCacccGUCUGAa-- -3' miRNA: 3'- uUGUGCAAUG------------CGCGUCG----CAGACUgug -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 43213 | 0.68 | 0.655867 |
Target: 5'- uACGCGcgGCGCGCucuuCGUC-GACGCg -3' miRNA: 3'- uUGUGCaaUGCGCGuc--GCAGaCUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 16207 | 0.68 | 0.644436 |
Target: 5'- -cCGgGUUACGCGCcG-GUCUGACGg -3' miRNA: 3'- uuGUgCAAUGCGCGuCgCAGACUGUg -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 43772 | 0.68 | 0.621549 |
Target: 5'- --aGCGUUggGCGCGCAGCaGUC-GACGu -3' miRNA: 3'- uugUGCAA--UGCGCGUCG-CAGaCUGUg -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 41004 | 0.68 | 0.621549 |
Target: 5'- cACGCGgaGCGCGCAcCGUCaUGGCu- -3' miRNA: 3'- uUGUGCaaUGCGCGUcGCAG-ACUGug -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 41962 | 0.69 | 0.598702 |
Target: 5'- cGGCGCGg---GgGCGGCGUCcGGCGCa -3' miRNA: 3'- -UUGUGCaaugCgCGUCGCAGaCUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 24530 | 0.69 | 0.598702 |
Target: 5'- uGACAUGg--UGCGCGGCGUCacuggccGGCGCg -3' miRNA: 3'- -UUGUGCaauGCGCGUCGCAGa------CUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 32622 | 0.69 | 0.575978 |
Target: 5'- cGCGCGUgACGCGguGCGUgcaGCGCa -3' miRNA: 3'- uUGUGCAaUGCGCguCGCAgacUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 5784 | 0.7 | 0.509276 |
Target: 5'- gGACAuuCGaUugGCccCGGCGUCUGACACu -3' miRNA: 3'- -UUGU--GCaAugCGc-GUCGCAGACUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 36880 | 0.7 | 0.509276 |
Target: 5'- uAGCGCGUUgcugcgGCGCGCAG-GUCUuucggGAUACg -3' miRNA: 3'- -UUGUGCAA------UGCGCGUCgCAGA-----CUGUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 32563 | 0.72 | 0.406393 |
Target: 5'- -cCGCGUcACGCGCGGCGguguucaCUGugACc -3' miRNA: 3'- uuGUGCAaUGCGCGUCGCa------GACugUG- -5' |
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27932 | 5' | -53.1 | NC_005886.2 | + | 46620 | 0.73 | 0.369039 |
Target: 5'- -uCGCGggUugGuCGCAGCGUgUGGCGCu -3' miRNA: 3'- uuGUGCa-AugC-GCGUCGCAgACUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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