Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27933 | 3' | -48.6 | NC_005886.2 | + | 36027 | 0.66 | 0.960819 |
Target: 5'- -cGaCGAacgAUCGGCGCuauacgauuauguacGGAUGGCGUGUc -3' miRNA: 3'- uaC-GCU---UAGCUGCGu--------------UCUACUGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 32989 | 0.66 | 0.956177 |
Target: 5'- -cGCGcGUUG-CGCGGGAaGACcUGCg -3' miRNA: 3'- uaCGCuUAGCuGCGUUCUaCUGuACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 21202 | 0.66 | 0.951644 |
Target: 5'- gGUGCGuGUCGuCGaCAaagacaaugaAGAUGuCGUGCa -3' miRNA: 3'- -UACGCuUAGCuGC-GU----------UCUACuGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 23347 | 0.66 | 0.946807 |
Target: 5'- cAUGCGAAUCuuuUGCAGGuccGGgAUGCg -3' miRNA: 3'- -UACGCUUAGcu-GCGUUCua-CUgUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 20834 | 0.67 | 0.936204 |
Target: 5'- uUGCGuucgaAAUgGGCGCGguuGAUGGCGUGg -3' miRNA: 3'- uACGC-----UUAgCUGCGUu--CUACUGUACg -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 38566 | 0.68 | 0.904192 |
Target: 5'- -aGCGAGcgCGACGgaCGGGAuaccgUGACGUGUg -3' miRNA: 3'- uaCGCUUa-GCUGC--GUUCU-----ACUGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 24076 | 0.68 | 0.904192 |
Target: 5'- -cGCGAcgAUCcGCGCaAAGAUGACGaGUg -3' miRNA: 3'- uaCGCU--UAGcUGCG-UUCUACUGUaCG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 44561 | 0.68 | 0.896853 |
Target: 5'- -aGCGAA-CGACGCAaucauuccGGGUGGCGg-- -3' miRNA: 3'- uaCGCUUaGCUGCGU--------UCUACUGUacg -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 32752 | 0.68 | 0.889209 |
Target: 5'- uUGCGAAU-GAUGCGGGAcagcgUGACAUa- -3' miRNA: 3'- uACGCUUAgCUGCGUUCU-----ACUGUAcg -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 15100 | 0.7 | 0.797691 |
Target: 5'- --uCGAcgCGACGUAGGcuguUGGCGUGCc -3' miRNA: 3'- uacGCUuaGCUGCGUUCu---ACUGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 25912 | 0.7 | 0.796656 |
Target: 5'- -cGCGGGcuugaauUCGACGCGGGcUGguuucGCAUGCa -3' miRNA: 3'- uaCGCUU-------AGCUGCGUUCuAC-----UGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 39851 | 0.7 | 0.787257 |
Target: 5'- -cGCGggUCGaagucGCGCAaagcGGGUGACGaaauuccgcUGCg -3' miRNA: 3'- uaCGCuuAGC-----UGCGU----UCUACUGU---------ACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 7213 | 0.7 | 0.776637 |
Target: 5'- aGUGCGAccauugauAUCGAUGCugAAGAcGaACAUGCa -3' miRNA: 3'- -UACGCU--------UAGCUGCG--UUCUaC-UGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 38280 | 0.72 | 0.675051 |
Target: 5'- cGUGCGAAcCGACGUgcGcgGGCGUGa -3' miRNA: 3'- -UACGCUUaGCUGCGuuCuaCUGUACg -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 25801 | 0.73 | 0.639859 |
Target: 5'- -cGCGuGUCGGCGCGuuuGGUGaaucugguGCAUGCg -3' miRNA: 3'- uaCGCuUAGCUGCGUu--CUAC--------UGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 6514 | 0.73 | 0.628096 |
Target: 5'- aAUGCaauacgCGAUGCAAGAcGGCAUGCc -3' miRNA: 3'- -UACGcuua--GCUGCGUUCUaCUGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 44532 | 0.74 | 0.592876 |
Target: 5'- -cGUGAucaacGUUGACGUGcGGAUGACGUGCu -3' miRNA: 3'- uaCGCU-----UAGCUGCGU-UCUACUGUACG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 43479 | 0.76 | 0.479356 |
Target: 5'- -gGCGAcggaAUCGACGCG-GAUGGCAaGCc -3' miRNA: 3'- uaCGCU----UAGCUGCGUuCUACUGUaCG- -5' |
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27933 | 3' | -48.6 | NC_005886.2 | + | 21008 | 1.12 | 0.00242 |
Target: 5'- aAUGCGAAUCGACGCAAGAUGACAUGCg -3' miRNA: 3'- -UACGCUUAGCUGCGUUCUACUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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