Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27933 | 5' | -52.6 | NC_005886.2 | + | 18749 | 0.67 | 0.74363 |
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Target: 5'- gCGGCaAUCAUCuuGCgucUGUggAUGgCGGUCg -3' miRNA: 3'- -GCCG-UAGUAGu-CGa--ACA--UACgGCCAG- -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 42038 | 0.68 | 0.699333 |
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Target: 5'- gCGGCGcgaacacuUCGuUCAGCgUGUcgGCCGG-Ca -3' miRNA: 3'- -GCCGU--------AGU-AGUCGaACAuaCGGCCaG- -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 23309 | 0.68 | 0.676709 |
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Target: 5'- cCGGuCGUCGaCGGUUUGUAgagcgGCUGGUg -3' miRNA: 3'- -GCC-GUAGUaGUCGAACAUa----CGGCCAg -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 19040 | 0.69 | 0.631007 |
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Target: 5'- uGGCAUCAUUucGCUUG-AUGCCauGGaUCg -3' miRNA: 3'- gCCGUAGUAGu-CGAACaUACGG--CC-AG- -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 41607 | 0.7 | 0.529209 |
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Target: 5'- uGuGCGUCGUCAGaCUUGuUGUGCaGGUUg -3' miRNA: 3'- gC-CGUAGUAGUC-GAAC-AUACGgCCAG- -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 20898 | 0.73 | 0.385644 |
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Target: 5'- cCGGCAUaca-AGCUgaugAUGCCGGUCg -3' miRNA: 3'- -GCCGUAguagUCGAaca-UACGGCCAG- -5' |
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| 27933 | 5' | -52.6 | NC_005886.2 | + | 20968 | 1.11 | 0.000962 |
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Target: 5'- cCGGCAUCAUCAGCUUGUAUGCCGGUCg -3' miRNA: 3'- -GCCGUAGUAGUCGAACAUACGGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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