Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27934 | 5' | -60.8 | NC_005886.2 | + | 24764 | 0.66 | 0.382654 |
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Target: 5'- cGUUGcGCGCGGaCGCGGG-GCUUcuaGCGGc -3' miRNA: 3'- -CAGC-UGCGCC-GCGUCCaCGAG---CGCUu -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 25805 | 0.66 | 0.373946 |
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Target: 5'- uGUCGGCGCguuuGGUGaaucuGGUGCaUGCGAAa -3' miRNA: 3'- -CAGCUGCG----CCGCgu---CCACGaGCGCUU- -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 15099 | 0.66 | 0.356937 |
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Target: 5'- aUCGACGCGaCGUAGGcUGUUgGCGu- -3' miRNA: 3'- cAGCUGCGCcGCGUCC-ACGAgCGCuu -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 39860 | 0.67 | 0.316834 |
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Target: 5'- uUCGACG-GGCGCGGGUcGaagUCGCGc- -3' miRNA: 3'- cAGCUGCgCCGCGUCCA-Cg--AGCGCuu -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 38931 | 0.67 | 0.309233 |
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Target: 5'- aUCGGCGCuGCuGCGGGcUGCUCGacaGGGg -3' miRNA: 3'- cAGCUGCGcCG-CGUCC-ACGAGCg--CUU- -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 1240 | 0.68 | 0.253416 |
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Target: 5'- cUCGugGCGGCGgGGGcuucgGCccucacuaUCGCGAc -3' miRNA: 3'- cAGCugCGCCGCgUCCa----CG--------AGCGCUu -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 1853 | 0.72 | 0.13417 |
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Target: 5'- -cCGGgGCGGUGUAGGUGCUguuuggUGCGAGc -3' miRNA: 3'- caGCUgCGCCGCGUCCACGA------GCGCUU- -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 7160 | 0.74 | 0.110602 |
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Target: 5'- cGUCGAuCGCcacGGUGCcGGUGCUCGCGc- -3' miRNA: 3'- -CAGCU-GCG---CCGCGuCCACGAGCGCuu -5' |
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| 27934 | 5' | -60.8 | NC_005886.2 | + | 21286 | 1.05 | 0.000433 |
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Target: 5'- gGUCGACGCGGCGCAGGUGCUCGCGAAc -3' miRNA: 3'- -CAGCUGCGCCGCGUCCACGAGCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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