miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27935 3' -51.7 NC_005886.2 + 16897 0.7 0.630444
Target:  5'- -uUCGAGCCGUGUacaGGUGUcgUCGg -3'
miRNA:   3'- guAGCUCGGCGCGcagCCAUAaaAGC- -5'
27935 3' -51.7 NC_005886.2 + 1513 0.73 0.476498
Target:  5'- gCGUCGAuuuGCCgGCGuCGUCGGUAUUcaucucgaaUUCGg -3'
miRNA:   3'- -GUAGCU---CGG-CGC-GCAGCCAUAA---------AAGC- -5'
27935 3' -51.7 NC_005886.2 + 25791 0.77 0.295242
Target:  5'- gCAUCGcGGCCGCGUGUCGGcgcgUUUGg -3'
miRNA:   3'- -GUAGC-UCGGCGCGCAGCCauaaAAGC- -5'
27935 3' -51.7 NC_005886.2 + 22138 1.1 0.001876
Target:  5'- gCAUCGAGCCGCGCGUCGGUAUUUUCGg -3'
miRNA:   3'- -GUAGCUCGGCGCGCAGCCAUAAAAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.