Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27937 | 3' | -53.2 | NC_005886.2 | + | 43131 | 0.66 | 0.739979 |
Target: 5'- aGGUUgcgcgCGUCGACGAAGa-GCGCGCc- -3' miRNA: 3'- gUCAA-----GCAGCUGCUUCgcCGUGUGaa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 42544 | 0.67 | 0.684664 |
Target: 5'- uCGGcgUCGUUGACGAu-CGGCACAUc- -3' miRNA: 3'- -GUCa-AGCAGCUGCUucGCCGUGUGaa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 31703 | 0.68 | 0.650731 |
Target: 5'- gCAGUUCGUCGGC-AGGCGGUccccgugaagAC-CUUg -3' miRNA: 3'- -GUCAAGCAGCUGcUUCGCCG----------UGuGAA- -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 23309 | 0.68 | 0.649596 |
Target: 5'- cCGG-UCGUCGACGGuuuguagAGCGGCugGu-- -3' miRNA: 3'- -GUCaAGCAGCUGCU-------UCGCCGugUgaa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 46564 | 0.68 | 0.627999 |
Target: 5'- aAGUUCGUCG-CGugaaauuuaacGAGCGGCAgACc- -3' miRNA: 3'- gUCAAGCAGCuGC-----------UUCGCCGUgUGaa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 37382 | 0.7 | 0.505322 |
Target: 5'- uCGGUgUCGUCGACGcguggcGCGGCGCAa-- -3' miRNA: 3'- -GUCA-AGCAGCUGCuu----CGCCGUGUgaa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 19754 | 0.71 | 0.463127 |
Target: 5'- -cGUUUGUCGAUGAucGGCGGUuCGCUc -3' miRNA: 3'- guCAAGCAGCUGCU--UCGCCGuGUGAa -5' |
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27937 | 3' | -53.2 | NC_005886.2 | + | 24242 | 1.06 | 0.002042 |
Target: 5'- cCAGUUCGUCGACGAAGCGGCACACUUg -3' miRNA: 3'- -GUCAAGCAGCUGCUUCGCCGUGUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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