Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27937 | 5' | -53.3 | NC_005886.2 | + | 13616 | 0.66 | 0.778847 |
Target: 5'- ---aGUGCGGCGgaUGuCGGCGcguacuucgguACGGCg -3' miRNA: 3'- caucUAUGCCGCgaAC-GCCGU-----------UGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 7142 | 0.66 | 0.747184 |
Target: 5'- --cGGUGCucGCGCUcgGUGGCGGCGGa -3' miRNA: 3'- cauCUAUGc-CGCGAa-CGCCGUUGCUg -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 38525 | 0.66 | 0.736363 |
Target: 5'- ---aGUGCGGCGCggcCGGCAACa-- -3' miRNA: 3'- caucUAUGCCGCGaacGCCGUUGcug -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 17775 | 0.66 | 0.72543 |
Target: 5'- aGgcGGUACGGgcgUGCUUGCcGGCAuucucGCGAa -3' miRNA: 3'- -CauCUAUGCC---GCGAACG-CCGU-----UGCUg -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 41969 | 0.66 | 0.72543 |
Target: 5'- --cGAcaauCGGCGCggggGCGGCGucCGGCg -3' miRNA: 3'- cauCUau--GCCGCGaa--CGCCGUu-GCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 43807 | 0.67 | 0.715505 |
Target: 5'- -aAGGUGCGGUGCaaUUGggaaugcagccggcaGGCAugGGCg -3' miRNA: 3'- caUCUAUGCCGCG--AACg--------------CCGUugCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 36296 | 0.67 | 0.692084 |
Target: 5'- -cGGcgGCGGCaucgGCGGCAucgGCGGCa -3' miRNA: 3'- caUCuaUGCCGcgaaCGCCGU---UGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 42442 | 0.67 | 0.692084 |
Target: 5'- gGUGGAUGC-GCGg-UGCGGCAACc-- -3' miRNA: 3'- -CAUCUAUGcCGCgaACGCCGUUGcug -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 38929 | 0.67 | 0.680829 |
Target: 5'- --cGAU-CGGCGCUgcUGCGGgCugcuCGACa -3' miRNA: 3'- cauCUAuGCCGCGA--ACGCC-Guu--GCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 18061 | 0.68 | 0.645674 |
Target: 5'- aUGGAUcgugaaAUGGUGCUUGUgcaggacGGCAAgGACa -3' miRNA: 3'- cAUCUA------UGCCGCGAACG-------CCGUUgCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 36365 | 0.68 | 0.635429 |
Target: 5'- -cGGcgGCGGCaCggGCGGCGGCaGGCg -3' miRNA: 3'- caUCuaUGCCGcGaaCGCCGUUG-CUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 35634 | 0.68 | 0.624042 |
Target: 5'- -----cGCGuGCGCUaUGCGGC-GCGGCg -3' miRNA: 3'- caucuaUGC-CGCGA-ACGCCGuUGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 27878 | 0.68 | 0.612664 |
Target: 5'- cGUAGAcaaAUaGCGCauaUGCGGCAGCGAg -3' miRNA: 3'- -CAUCUa--UGcCGCGa--ACGCCGUUGCUg -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 936 | 0.69 | 0.601303 |
Target: 5'- ----uUACGGCuGCacgUUGCGGCuuCGACa -3' miRNA: 3'- caucuAUGCCG-CG---AACGCCGuuGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 10329 | 0.69 | 0.589971 |
Target: 5'- -----cACGGgGCUUGCGGCAGuuGCa -3' miRNA: 3'- caucuaUGCCgCGAACGCCGUUgcUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 43910 | 0.69 | 0.589971 |
Target: 5'- -aGGAUG-GGCGCaccgGCaGGCAugGGCg -3' miRNA: 3'- caUCUAUgCCGCGaa--CG-CCGUugCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 36197 | 0.69 | 0.545112 |
Target: 5'- -cGGcgGCGGCaCggGCGGCAucgGCGGCg -3' miRNA: 3'- caUCuaUGCCGcGaaCGCCGU---UGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 1724 | 0.73 | 0.337154 |
Target: 5'- ----uUGCGGCGggUGCGGCAGUGGCa -3' miRNA: 3'- caucuAUGCCGCgaACGCCGUUGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 22652 | 0.74 | 0.304639 |
Target: 5'- -gAGAuUGCGGCGaaugcUGCGGCGAUGAUg -3' miRNA: 3'- caUCU-AUGCCGCga---ACGCCGUUGCUG- -5' |
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27937 | 5' | -53.3 | NC_005886.2 | + | 36248 | 0.75 | 0.267455 |
Target: 5'- -cGGcgGCGGCaCggGCGGCGGCGGCg -3' miRNA: 3'- caUCuaUGCCGcGaaCGCCGUUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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