Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27938 | 3' | -53.2 | NC_005886.2 | + | 31472 | 0.66 | 0.763349 |
Target: 5'- cGGacaACGGUAUCugGCGGCGaauCAUGa -3' miRNA: 3'- cUCg--UGCCAUAGugCGUUGCgc-GUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 41208 | 0.66 | 0.759086 |
Target: 5'- cAGCACGGgcgacgaagaaccGUUGCGCGAUGCGUc-- -3' miRNA: 3'- cUCGUGCCa------------UAGUGCGUUGCGCGuau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 20822 | 0.66 | 0.746168 |
Target: 5'- uGGGCGCGGUugAUgGCGUGGaugugaacgaauaccUGCGCAUGg -3' miRNA: 3'- -CUCGUGCCA--UAgUGCGUU---------------GCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 24522 | 0.67 | 0.708661 |
Target: 5'- -uGCGCGGcGUCACuggcCGGCGCGCu-- -3' miRNA: 3'- cuCGUGCCaUAGUGc---GUUGCGCGuau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 40285 | 0.67 | 0.686126 |
Target: 5'- --uCGCGGUcgacaucgagCAUGCGACGCGCAUu -3' miRNA: 3'- cucGUGCCAua--------GUGCGUUGCGCGUAu -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 33060 | 0.67 | 0.674767 |
Target: 5'- -cGCA-GGUcuuccCGCGCAACGCGCGa- -3' miRNA: 3'- cuCGUgCCAua---GUGCGUUGCGCGUau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 35662 | 0.67 | 0.674767 |
Target: 5'- uGGGC-CGGUAUCcCGCcgcuuuACGUGCAg- -3' miRNA: 3'- -CUCGuGCCAUAGuGCGu-----UGCGCGUau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 19959 | 0.67 | 0.663366 |
Target: 5'- aGGCguucaACGGcAUCaACGCAGgGCGCAUGc -3' miRNA: 3'- cUCG-----UGCCaUAG-UGCGUUgCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 32186 | 0.68 | 0.651932 |
Target: 5'- aGGUACGcGUAcggcUCGCGCAguGgGCGCGUGa -3' miRNA: 3'- cUCGUGC-CAU----AGUGCGU--UgCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 37401 | 0.68 | 0.630164 |
Target: 5'- cGGGCugcuucaucuacaauCGGUGUCgucgACGCGugGCGCGg- -3' miRNA: 3'- -CUCGu--------------GCCAUAG----UGCGUugCGCGUau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 38367 | 0.68 | 0.606115 |
Target: 5'- cGAGCGCGuucucAUCACGCc-CGCGCAc- -3' miRNA: 3'- -CUCGUGCca---UAGUGCGuuGCGCGUau -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 15808 | 0.69 | 0.571967 |
Target: 5'- cGGCGCGGacggAUCGCGCuccGACGUGUGUu -3' miRNA: 3'- cUCGUGCCa---UAGUGCG---UUGCGCGUAu -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 45990 | 0.7 | 0.494534 |
Target: 5'- -cGUACGGgaAUguCGCGACGUGCGUAg -3' miRNA: 3'- cuCGUGCCa-UAguGCGUUGCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 14391 | 0.7 | 0.483844 |
Target: 5'- cAGUugGGg--CAcCGCAACGUGCGUGu -3' miRNA: 3'- cUCGugCCauaGU-GCGUUGCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 35505 | 0.71 | 0.442254 |
Target: 5'- cGGGCGCGcaGUAUCGUGCGuCGCGCAUu -3' miRNA: 3'- -CUCGUGC--CAUAGUGCGUuGCGCGUAu -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 39855 | 0.73 | 0.35666 |
Target: 5'- cGGGCGCGGgucgaaGUCGCGCAAaGCGgGUGa -3' miRNA: 3'- -CUCGUGCCa-----UAGUGCGUUgCGCgUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 5591 | 0.75 | 0.268818 |
Target: 5'- ---gACGGUAUCACGCugguUGCGCAUAg -3' miRNA: 3'- cucgUGCCAUAGUGCGuu--GCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 9673 | 0.78 | 0.167877 |
Target: 5'- aGAGUggugcacGCGGUAUCGCGCAAuggcuugguuCGCGCGUGa -3' miRNA: 3'- -CUCG-------UGCCAUAGUGCGUU----------GCGCGUAU- -5' |
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27938 | 3' | -53.2 | NC_005886.2 | + | 25781 | 1.06 | 0.00172 |
Target: 5'- uGAGCACGGUAUCACGCAACGCGCAUAc -3' miRNA: 3'- -CUCGUGCCAUAGUGCGUUGCGCGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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