Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27938 | 5' | -57.4 | NC_005886.2 | + | 17814 | 0.66 | 0.546539 |
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Target: 5'- gGCUUUGCUCgGC-GCGAGCcgugUUUgCGGGa -3' miRNA: 3'- -CGGAGCGAG-CGaCGCUCG----AAAgGCCU- -5' |
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| 27938 | 5' | -57.4 | NC_005886.2 | + | 11003 | 0.67 | 0.503298 |
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Target: 5'- gGCCUUGCaagCGCUGUucGAGCUUgucgcuaaguuugUCCGa- -3' miRNA: 3'- -CGGAGCGa--GCGACG--CUCGAA-------------AGGCcu -5' |
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| 27938 | 5' | -57.4 | NC_005886.2 | + | 26025 | 0.67 | 0.473628 |
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Target: 5'- uGCggCGUUaCGCUGCGGGCgugaugucaCCGGAu -3' miRNA: 3'- -CGgaGCGA-GCGACGCUCGaaa------GGCCU- -5' |
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| 27938 | 5' | -57.4 | NC_005886.2 | + | 46488 | 0.67 | 0.473628 |
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Target: 5'- aGCCUUGCUCGUaguggaUGCGAuGCUgugCCa-- -3' miRNA: 3'- -CGGAGCGAGCG------ACGCU-CGAaa-GGccu -5' |
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| 27938 | 5' | -57.4 | NC_005886.2 | + | 37410 | 0.73 | 0.213125 |
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Target: 5'- cCCUCcaUCGUUGCGAGCcUUUCCGGc -3' miRNA: 3'- cGGAGcgAGCGACGCUCG-AAAGGCCu -5' |
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| 27938 | 5' | -57.4 | NC_005886.2 | + | 25745 | 1.11 | 0.000358 |
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Target: 5'- cGCCUCGCUCGCUGCGAGCUUUCCGGAa -3' miRNA: 3'- -CGGAGCGAGCGACGCUCGAAAGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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