Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27939 | 5' | -46.4 | NC_005886.2 | + | 15921 | 0.66 | 0.993323 |
Target: 5'- ---aCUGU-UCGAGAuguUCGAgCGCCu -3' miRNA: 3'- gauaGACGuAGCUCUuuuAGCU-GCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 26180 | 0.66 | 0.993323 |
Target: 5'- ---cCUGUAUCG-GAAGGU-GACGCg -3' miRNA: 3'- gauaGACGUAGCuCUUUUAgCUGCGg -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 9519 | 0.66 | 0.993323 |
Target: 5'- -----aGCGauUCGGGccacGUCGACGCCg -3' miRNA: 3'- gauagaCGU--AGCUCuuu-UAGCUGCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 44906 | 0.66 | 0.989435 |
Target: 5'- aUAUCUGCAuacgauUCGuGGu--UCGAUGCg -3' miRNA: 3'- gAUAGACGU------AGCuCUuuuAGCUGCGg -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 7223 | 0.67 | 0.985989 |
Target: 5'- -cAUCUGCAUUGAcuguuugcGAAAuAUCGugcgaaguACGCCg -3' miRNA: 3'- gaUAGACGUAGCU--------CUUU-UAGC--------UGCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 2404 | 0.67 | 0.983972 |
Target: 5'- gUGUC-GCGUcCGGGAcggcAGAUUGAuCGCCa -3' miRNA: 3'- gAUAGaCGUA-GCUCU----UUUAGCU-GCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 15746 | 0.68 | 0.976594 |
Target: 5'- aCUG-CUGCAUcCGGGucuucuUCGGCGCg -3' miRNA: 3'- -GAUaGACGUA-GCUCuuuu--AGCUGCGg -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 28801 | 0.68 | 0.970454 |
Target: 5'- ---aUUGgAUCGAGA----CGACGCCa -3' miRNA: 3'- gauaGACgUAGCUCUuuuaGCUGCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 23961 | 0.69 | 0.950248 |
Target: 5'- uUAUCccGC-UCGAauGGAUCGAUGCCg -3' miRNA: 3'- gAUAGa-CGuAGCUcuUUUAGCUGCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 40130 | 0.7 | 0.934507 |
Target: 5'- -gAUCUGU-UCGGGGAAGgcuUCGACcuGCCg -3' miRNA: 3'- gaUAGACGuAGCUCUUUU---AGCUG--CGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 44195 | 0.7 | 0.928631 |
Target: 5'- --uUCUGCGUCGGcacGAGuugCGGCGCa -3' miRNA: 3'- gauAGACGUAGCU---CUUuuaGCUGCGg -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 40530 | 0.7 | 0.922439 |
Target: 5'- gUAUCUGCGaCGcGAuGAUCGAagggcCGCCg -3' miRNA: 3'- gAUAGACGUaGCuCUuUUAGCU-----GCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 7737 | 0.72 | 0.861769 |
Target: 5'- -gGUCUcGCAUCaAGGAAAUCGAacauauUGCCg -3' miRNA: 3'- gaUAGA-CGUAGcUCUUUUAGCU------GCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 37119 | 0.77 | 0.61223 |
Target: 5'- ---gCUGCAUCGAGA--AUCaACGCCu -3' miRNA: 3'- gauaGACGUAGCUCUuuUAGcUGCGG- -5' |
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27939 | 5' | -46.4 | NC_005886.2 | + | 27266 | 1.14 | 0.003831 |
Target: 5'- gCUAUCUGCAUCGAGAAAAUCGACGCCu -3' miRNA: 3'- -GAUAGACGUAGCUCUUUUAGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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