Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2794 | 5' | -51.1 | NC_001491.2 | + | 32249 | 0.7 | 0.916014 |
Target: 5'- cGCUGuUGCUGGGGgUGuGCAA-GAGGg -3' miRNA: 3'- -CGAUuGCGAUCCCgAU-CGUUaCUCCg -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 57403 | 0.7 | 0.906008 |
Target: 5'- aGCUAgcuugaucaugcacuACGCUggugGGGGCUgAGCA--GGGGCu -3' miRNA: 3'- -CGAU---------------UGCGA----UCCCGA-UCGUuaCUCCG- -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 13939 | 0.75 | 0.665305 |
Target: 5'- cGCUGguauagcGCGCcGGGGCUGGCGGccuUG-GGCg -3' miRNA: 3'- -CGAU-------UGCGaUCCCGAUCGUU---ACuCCG- -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 112435 | 0.77 | 0.602296 |
Target: 5'- gGCUAGgGCagGGGGCUagGGCAG-GGGGCu -3' miRNA: 3'- -CGAUUgCGa-UCCCGA--UCGUUaCUCCG- -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 112474 | 0.77 | 0.602296 |
Target: 5'- gGCUAGgGCagGGGGCUagGGCAG-GGGGCu -3' miRNA: 3'- -CGAUUgCGa-UCCCGA--UCGUUaCUCCG- -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 112513 | 0.77 | 0.602296 |
Target: 5'- gGCUAGgGCagGGGGCUagGGCAG-GGGGCu -3' miRNA: 3'- -CGAUUgCGa-UCCCGA--UCGUUaCUCCG- -5' |
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2794 | 5' | -51.1 | NC_001491.2 | + | 80357 | 0.84 | 0.276625 |
Target: 5'- cGCggguCGCUGGGGCUGGU--UGGGGCa -3' miRNA: 3'- -CGauu-GCGAUCCCGAUCGuuACUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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