Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27942 | 3' | -58.2 | NC_005886.2 | + | 47270 | 0.66 | 0.53267 |
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Target: 5'- cCCGUugCCgaCGAagguGUGCCCGAAcUGCu -3' miRNA: 3'- cGGCGugGG--GCU----UACGGGCUUcACGc -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 4710 | 0.66 | 0.520126 |
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Target: 5'- aGCCGaCGCCggcuugugacgaaCCGAaauuaccGUGUCCGAAGUucgcGCGg -3' miRNA: 3'- -CGGC-GUGG-------------GGCU-------UACGGGCUUCA----CGC- -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 44268 | 0.68 | 0.39535 |
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Target: 5'- -aUGCGCCCCGuuucGUuuGGAGUGUGu -3' miRNA: 3'- cgGCGUGGGGCuua-CGggCUUCACGC- -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 2228 | 0.68 | 0.385547 |
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Target: 5'- gGCCGUaACCCauucggGAAUGCCgguuuuuUGAAGUGCa -3' miRNA: 3'- -CGGCG-UGGGg-----CUUACGG-------GCUUCACGc -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 29723 | 0.69 | 0.377647 |
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Target: 5'- cGUCGCACUUCGg--GCauuCGggGUGCGg -3' miRNA: 3'- -CGGCGUGGGGCuuaCGg--GCuuCACGC- -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 42454 | 0.77 | 0.108552 |
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Target: 5'- cGCCGCACUCCGggUGgaUgCGcGGUGCGg -3' miRNA: 3'- -CGGCGUGGGGCuuAC--GgGCuUCACGC- -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 42377 | 0.79 | 0.077455 |
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Target: 5'- uGCCGCACCgCGcAUccaCCCGGAGUGCGg -3' miRNA: 3'- -CGGCGUGGgGCuUAc--GGGCUUCACGC- -5' |
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| 27942 | 3' | -58.2 | NC_005886.2 | + | 29798 | 1.11 | 0.000322 |
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Target: 5'- cGCCGCACCCCGAAUGCCCGAAGUGCGa -3' miRNA: 3'- -CGGCGUGGGGCUUACGGGCUUCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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