Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27942 | 5' | -53.9 | NC_005886.2 | + | 41950 | 0.66 | 0.747096 |
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Target: 5'- gGCGUCCGGCGcaggCAUUGCGaccauGACGg -3' miRNA: 3'- aUGCGGGCUGCacaaGUAACGC-----CUGU- -5' |
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| 27942 | 5' | -53.9 | NC_005886.2 | + | 9435 | 0.66 | 0.725531 |
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Target: 5'- aACGCUCGuCGUGUggCGUUG-GGugAa -3' miRNA: 3'- aUGCGGGCuGCACAa-GUAACgCCugU- -5' |
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| 27942 | 5' | -53.9 | NC_005886.2 | + | 7994 | 0.67 | 0.681323 |
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Target: 5'- gUACGUUgGGCGUGUUagcaGCGGGCu -3' miRNA: 3'- -AUGCGGgCUGCACAAguaaCGCCUGu -5' |
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| 27942 | 5' | -53.9 | NC_005886.2 | + | 12663 | 0.68 | 0.602489 |
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Target: 5'- aAUGCCCGACaaaGUGUcaUCGUUGaCGGcCGg -3' miRNA: 3'- aUGCGGGCUG---CACA--AGUAAC-GCCuGU- -5' |
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| 27942 | 5' | -53.9 | NC_005886.2 | + | 46760 | 0.69 | 0.524892 |
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Target: 5'- cACGCCgGACG-GUcgcgaUAUUGCGGACc -3' miRNA: 3'- aUGCGGgCUGCaCAa----GUAACGCCUGu -5' |
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| 27942 | 5' | -53.9 | NC_005886.2 | + | 29762 | 1.07 | 0.001559 |
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Target: 5'- gUACGCCCGACGUGUUCAUUGCGGACAc -3' miRNA: 3'- -AUGCGGGCUGCACAAGUAACGCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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