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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27945 | 3' | -51.5 | NC_005886.2 | + | 25776 | 0.66 | 0.851506 |
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Target: 5'- aCGGUAUcacGCAACGCGCAUacgcgGAAuacgccucgcucGCUGc -3' miRNA: 3'- -GCCAUA---CGUUGCGCGUGa----CUU------------CGAUa -5' |
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| 27945 | 3' | -51.5 | NC_005886.2 | + | 14541 | 0.68 | 0.728923 |
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Target: 5'- uCGGaaccgGCGACGCGUAC-GAGGCg-- -3' miRNA: 3'- -GCCaua--CGUUGCGCGUGaCUUCGaua -5' |
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| 27945 | 3' | -51.5 | NC_005886.2 | + | 23693 | 0.72 | 0.50036 |
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Target: 5'- aGGcGUGCGugGCGaUGCUGAGGCa-- -3' miRNA: 3'- gCCaUACGUugCGC-GUGACUUCGaua -5' |
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| 27945 | 3' | -51.5 | NC_005886.2 | + | 40283 | 0.74 | 0.378055 |
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Target: 5'- gCGGUcgacaucgagcAUGCGACGCGCAUuugUGAAGCc-- -3' miRNA: 3'- -GCCA-----------UACGUUGCGCGUG---ACUUCGaua -5' |
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| 27945 | 3' | -51.5 | NC_005886.2 | + | 35570 | 1.06 | 0.002837 |
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Target: 5'- uCGGUAUGCAACGCGCACUGAAGCUAUu -3' miRNA: 3'- -GCCAUACGUUGCGCGUGACUUCGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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