Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27946 | 3' | -52.9 | NC_005886.2 | + | 16654 | 0.66 | 0.805303 |
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Target: 5'- uGCGcccacaGCGGGGAgccGCCCGCAccGUUCg -3' miRNA: 3'- uUGCuc----UGCCUCU---UGGGCGUuaCAGG- -5' |
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| 27946 | 3' | -52.9 | NC_005886.2 | + | 35278 | 0.66 | 0.805303 |
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Target: 5'- -uCGGuGACGGAcGAcGCCgGCAAUGUgCa -3' miRNA: 3'- uuGCU-CUGCCU-CU-UGGgCGUUACAgG- -5' |
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| 27946 | 3' | -52.9 | NC_005886.2 | + | 26665 | 0.67 | 0.754205 |
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Target: 5'- cAACGcGACGGGGAguuACCaauGCGAUcaaccGUCCg -3' miRNA: 3'- -UUGCuCUGCCUCU---UGGg--CGUUA-----CAGG- -5' |
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| 27946 | 3' | -52.9 | NC_005886.2 | + | 37102 | 0.68 | 0.688695 |
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Target: 5'- aGGCGuGACGGcAGGACUCGuCGGcGUCUa -3' miRNA: 3'- -UUGCuCUGCC-UCUUGGGC-GUUaCAGG- -5' |
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| 27946 | 3' | -52.9 | NC_005886.2 | + | 46979 | 0.7 | 0.586173 |
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Target: 5'- gGACGAuGCGGaAGAuggucauccaucaACCCcuuGCGAUGUCCu -3' miRNA: 3'- -UUGCUcUGCC-UCU-------------UGGG---CGUUACAGG- -5' |
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| 27946 | 3' | -52.9 | NC_005886.2 | + | 38689 | 1.09 | 0.001482 |
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Target: 5'- cAACGAGACGGAGAACCCGCAAUGUCCc -3' miRNA: 3'- -UUGCUCUGCCUCUUGGGCGUUACAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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