Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27946 | 5' | -54.7 | NC_005886.2 | + | 31936 | 0.65 | 0.759638 |
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Target: 5'- aGCGCGCGUUGCG--AUCCgGUgcgagcgcagcacCCGUCu -3' miRNA: 3'- -CGUGUGCAGUGCcaUAGGgCA-------------GGCAG- -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 7385 | 0.66 | 0.718674 |
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Target: 5'- -gGCACGUCACGGgacgcgaCCUGaaagCCGUa -3' miRNA: 3'- cgUGUGCAGUGCCaua----GGGCa---GGCAg -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 27716 | 0.66 | 0.718674 |
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Target: 5'- gGCGCAUcUUACGGUGcgcCCCGagUGUCg -3' miRNA: 3'- -CGUGUGcAGUGCCAUa--GGGCagGCAG- -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 35667 | 0.67 | 0.697116 |
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Target: 5'- aCGCAUGggcCGGUAUCCCG-CCGc- -3' miRNA: 3'- cGUGUGCaguGCCAUAGGGCaGGCag -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 18637 | 0.69 | 0.533118 |
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Target: 5'- uGCGCAUccaUCACGGcaaUAUCCgGUCCGg- -3' miRNA: 3'- -CGUGUGc--AGUGCC---AUAGGgCAGGCag -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 835 | 0.7 | 0.522492 |
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Target: 5'- -aACAauUGUCGgGGUAUCCCGcUCCGa- -3' miRNA: 3'- cgUGU--GCAGUgCCAUAGGGC-AGGCag -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 46061 | 0.72 | 0.393783 |
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Target: 5'- aCGCACGUCGCGacauUCCCGUaCgGUCu -3' miRNA: 3'- cGUGUGCAGUGCcau-AGGGCA-GgCAG- -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 14321 | 0.72 | 0.384751 |
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Target: 5'- aCGCACGUUGCGGUGcCCCaaCUGUCu -3' miRNA: 3'- cGUGUGCAGUGCCAUaGGGcaGGCAG- -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 29509 | 0.73 | 0.341695 |
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Target: 5'- gGCGCAgCGUUgACgGGUGUUCCGUCCGg- -3' miRNA: 3'- -CGUGU-GCAG-UG-CCAUAGGGCAGGCag -5' |
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| 27946 | 5' | -54.7 | NC_005886.2 | + | 38647 | 1.12 | 0.000682 |
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Target: 5'- gGCACACGUCACGGUAUCCCGUCCGUCg -3' miRNA: 3'- -CGUGUGCAGUGCCAUAGGGCAGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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