Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27952 | 3' | -52.3 | NC_005886.2 | + | 43835 | 0.73 | 0.415515 |
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Target: 5'- uGCUGCGcgCCCAA---CGCUGCGCCa -3' miRNA: 3'- cCGACGUaaGGGUUaacGUGGCGUGG- -5' |
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| 27952 | 3' | -52.3 | NC_005886.2 | + | 32534 | 0.73 | 0.405855 |
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Target: 5'- cGCUGCAaaCCCugcaUGCGCUGCACg -3' miRNA: 3'- cCGACGUaaGGGuua-ACGUGGCGUGg -5' |
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| 27952 | 3' | -52.3 | NC_005886.2 | + | 44117 | 0.73 | 0.405855 |
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Target: 5'- uGCUGCGgaaCgCCGGccgcUUGCAUCGCACCu -3' miRNA: 3'- cCGACGUaa-G-GGUU----AACGUGGCGUGG- -5' |
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| 27952 | 3' | -52.3 | NC_005886.2 | + | 37278 | 0.76 | 0.286329 |
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Target: 5'- cGGgUGCGgacgUgCCGAcgacgaaguaUUGCGCCGCGCCa -3' miRNA: 3'- -CCgACGUa---AgGGUU----------AACGUGGCGUGG- -5' |
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| 27952 | 3' | -52.3 | NC_005886.2 | + | 28347 | 0.77 | 0.264627 |
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Target: 5'- uGGCUGUgguacgauGUUCCCAAcgGUugCGCACa -3' miRNA: 3'- -CCGACG--------UAAGGGUUaaCGugGCGUGg -5' |
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| 27952 | 3' | -52.3 | NC_005886.2 | + | 43881 | 1.15 | 0.000605 |
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Target: 5'- cGGCUGCAUUCCCAAUUGCACCGCACCu -3' miRNA: 3'- -CCGACGUAAGGGUUAACGUGGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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