Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27952 | 5' | -59.4 | NC_005886.2 | + | 29774 | 0.66 | 0.419374 |
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Target: 5'- gCGACgGCgaagGUaCGCCCGACGUguucauUGCGg -3' miRNA: 3'- aGCUGaCGa---CGcGCGGGUUGCG------ACGC- -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 40228 | 0.67 | 0.410103 |
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Target: 5'- gUCGACcGCgaaCGUaaCCCAACGCUGCc -3' miRNA: 3'- -AGCUGaCGac-GCGc-GGGUUGCGACGc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 43907 | 0.67 | 0.391947 |
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Target: 5'- aUGcCUGCcgGUGCGCCCAugccuGCcgGCUGCa -3' miRNA: 3'- aGCuGACGa-CGCGCGGGU-----UG--CGACGc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 41572 | 0.67 | 0.383067 |
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Target: 5'- gUCGGC-GUca-GCGCCgGACGUUGCGg -3' miRNA: 3'- -AGCUGaCGacgCGCGGgUUGCGACGC- -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 41964 | 0.68 | 0.348911 |
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Target: 5'- aUCGGC-GCggggGCgGCGUCCGGCGCagGCa -3' miRNA: 3'- -AGCUGaCGa---CG-CGCGGGUUGCGa-CGc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 35436 | 0.69 | 0.301862 |
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Target: 5'- gCGACgcacgauaCUGCGCGCCCGuugcacccggcGCGCUGa- -3' miRNA: 3'- aGCUGac------GACGCGCGGGU-----------UGCGACgc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 1253 | 0.7 | 0.266512 |
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Target: 5'- aCGAgUGCU-UGCGCUCGugGCgGCGg -3' miRNA: 3'- aGCUgACGAcGCGCGGGUugCGaCGC- -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 32538 | 0.7 | 0.234587 |
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Target: 5'- aUCGACUGUUGCgGCGCUUGACGaugGUa -3' miRNA: 3'- -AGCUGACGACG-CGCGGGUUGCga-CGc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 24519 | 0.71 | 0.200582 |
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Target: 5'- gCGGCgucacugGCcgGCGCGCUCG-CGCUGCa -3' miRNA: 3'- aGCUGa------CGa-CGCGCGGGUuGCGACGc -5' |
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| 27952 | 5' | -59.4 | NC_005886.2 | + | 43841 | 1.08 | 0.000365 |
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Target: 5'- gUCGACUGCUGCGCGCCCAACGCUGCGc -3' miRNA: 3'- -AGCUGACGACGCGCGGGUUGCGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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