Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27954 | 5' | -58.6 | NC_005886.2 | + | 24445 | 0.71 | 0.249437 |
Target: 5'- aGCGcGAgcGCGCCGGCCaGUgacGCcgCGCAc -3' miRNA: 3'- -CGC-CU--UGCGGCCGG-CGaa-CGuaGCGU- -5' |
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27954 | 5' | -58.6 | NC_005886.2 | + | 19280 | 0.74 | 0.142719 |
Target: 5'- aCGGuuUGCCGucGCCGC-UGCAUCGCGa -3' miRNA: 3'- cGCCuuGCGGC--CGGCGaACGUAGCGU- -5' |
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27954 | 5' | -58.6 | NC_005886.2 | + | 19569 | 0.75 | 0.135088 |
Target: 5'- cGuuGAACGuCCGGCCGCUgGCGUUGUg -3' miRNA: 3'- -CgcCUUGC-GGCCGGCGAaCGUAGCGu -5' |
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27954 | 5' | -58.6 | NC_005886.2 | + | 44217 | 0.78 | 0.079319 |
Target: 5'- uUGGAGCguguaGCCGGCCGCUuucUGCGUCgGCAc -3' miRNA: 3'- cGCCUUG-----CGGCCGGCGA---ACGUAG-CGU- -5' |
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27954 | 5' | -58.6 | NC_005886.2 | + | 44114 | 1.09 | 0.000354 |
Target: 5'- uGCGGAACGCCGGCCGCUUGCAUCGCAc -3' miRNA: 3'- -CGCCUUGCGGCCGGCGAACGUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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