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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27956 | 5' | -55.7 | NC_005886.2 | + | 1493 | 1.09 | 0.000684 |
Target: 5'- gAGCGCCAGUGGCACCUUAAGCGUCGAu -3' miRNA: 3'- -UCGCGGUCACCGUGGAAUUCGCAGCU- -5' |
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27956 | 5' | -55.7 | NC_005886.2 | + | 43176 | 0.71 | 0.351167 |
Target: 5'- aAGCccuGCCGGUGGacgauCGCUUaGGGCGUCGAu -3' miRNA: 3'- -UCG---CGGUCACC-----GUGGAaUUCGCAGCU- -5' |
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27956 | 5' | -55.7 | NC_005886.2 | + | 7082 | 0.7 | 0.37744 |
Target: 5'- gAGCGCgAGcaccGGCACCgu-GGCGaUCGAc -3' miRNA: 3'- -UCGCGgUCa---CCGUGGaauUCGC-AGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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