Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27958 | 3' | -55.3 | NC_005886.2 | + | 22634 | 0.66 | 0.679918 |
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Target: 5'- uGaGCCUGUGcGGugAGUgagaUUGCGGCGAa -3' miRNA: 3'- gC-CGGACGC-CUugUCG----AACGCUGUUc -5' |
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| 27958 | 3' | -55.3 | NC_005886.2 | + | 36253 | 0.66 | 0.679918 |
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Target: 5'- gCGGCacggGCGGcGGCGGCggcacggGCGGCAc- -3' miRNA: 3'- -GCCGga--CGCC-UUGUCGaa-----CGCUGUuc -5' |
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| 27958 | 3' | -55.3 | NC_005886.2 | + | 42248 | 0.66 | 0.635261 |
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Target: 5'- uGGUCaGCGcGuGCAGCUUGaCGGCAu- -3' miRNA: 3'- gCCGGaCGC-CuUGUCGAAC-GCUGUuc -5' |
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| 27958 | 3' | -55.3 | NC_005886.2 | + | 38789 | 0.67 | 0.568373 |
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Target: 5'- cCGGCgagggUGCGGGAUgaAGCUUGCG-CAGu -3' miRNA: 3'- -GCCGg----ACGCCUUG--UCGAACGCuGUUc -5' |
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| 27958 | 3' | -55.3 | NC_005886.2 | + | 32759 | 0.68 | 0.544229 |
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Target: 5'- aCGGCauuugcgaaugaUGCGGGACAGC--GUGACAu- -3' miRNA: 3'- -GCCGg-----------ACGCCUUGUCGaaCGCUGUuc -5' |
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| 27958 | 3' | -55.3 | NC_005886.2 | + | 5283 | 1.09 | 0.000764 |
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Target: 5'- aCGGCCUGCGGAACAGCUUGCGACAAGu -3' miRNA: 3'- -GCCGGACGCCUUGUCGAACGCUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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