Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2796 | 3' | -60.9 | NC_001491.2 | + | 146820 | 0.66 | 0.72321 |
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Target: 5'- cGCaCCGCgaGGCgcaGCUCGUcGGAGCc- -3' miRNA: 3'- cCG-GGCG--CCGaagCGAGCA-CCUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 144608 | 0.66 | 0.713599 |
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Target: 5'- aGGUCCGgGGCgaagUCGCUgCGcu-GGCUGg -3' miRNA: 3'- -CCGGGCgCCGa---AGCGA-GCaccUCGAC- -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 115069 | 0.66 | 0.674587 |
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Target: 5'- gGGCuCCGCcaGGCgcaaGCUCcUGGuGCUGg -3' miRNA: 3'- -CCG-GGCG--CCGaag-CGAGcACCuCGAC- -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 148297 | 0.67 | 0.654854 |
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Target: 5'- aGGaCCgCGCGGCUgagguggCGCUCccGGGGCc- -3' miRNA: 3'- -CC-GG-GCGCCGAa------GCGAGcaCCUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 144478 | 0.67 | 0.644958 |
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Target: 5'- gGGuUCCGCGGCgggCGCUCGgacgacGGGCg- -3' miRNA: 3'- -CC-GGGCGCCGaa-GCGAGCac----CUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 147494 | 0.67 | 0.605351 |
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Target: 5'- uGGCCCGgGGUggccacccugCGCUUG-GGGGCc- -3' miRNA: 3'- -CCGGGCgCCGaa--------GCGAGCaCCUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 26911 | 0.68 | 0.585632 |
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Target: 5'- aGGCgCGgGGCUccaucaagCGCgcuccagcgCGUGGAGCUa -3' miRNA: 3'- -CCGgGCgCCGAa-------GCGa--------GCACCUCGAc -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 139992 | 0.7 | 0.471492 |
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Target: 5'- cGGaCCCGCuGCgccgCGC-CGUGGGGCg- -3' miRNA: 3'- -CC-GGGCGcCGaa--GCGaGCACCUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 114568 | 0.71 | 0.418731 |
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Target: 5'- cGCCUGCGGCcggcgcggCGCUggggcCGUGGAGCc- -3' miRNA: 3'- cCGGGCGCCGaa------GCGA-----GCACCUCGac -5' |
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| 2796 | 3' | -60.9 | NC_001491.2 | + | 115456 | 0.77 | 0.177135 |
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Target: 5'- cGGCCCGggaCGGCgcgCGCuUCGgGGAGCUGg -3' miRNA: 3'- -CCGGGC---GCCGaa-GCG-AGCaCCUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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