Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2796 | 5' | -52.9 | NC_001491.2 | + | 90268 | 0.66 | 0.959956 |
Target: 5'- cCCGaUGCCGaggcuAGCUUGCGCGCGc- -3' miRNA: 3'- cGGUcACGGUau---UUGGACGCGCGUuu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 6280 | 0.67 | 0.942993 |
Target: 5'- -aCGGUGCUGgcuAACCUGUGUGgGAAa -3' miRNA: 3'- cgGUCACGGUau-UUGGACGCGCgUUU- -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 121895 | 0.67 | 0.942993 |
Target: 5'- uUCAGUGCCcgcAGCgUgGCGCGCAGc -3' miRNA: 3'- cGGUCACGGuauUUGgA-CGCGCGUUu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 5669 | 0.67 | 0.932983 |
Target: 5'- aGCCGGaGCCGgaaaacguGCCgGCGCGCc-- -3' miRNA: 3'- -CGGUCaCGGUauu-----UGGaCGCGCGuuu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 140090 | 0.67 | 0.927588 |
Target: 5'- cGCCGG-GCCGcccgcggAGGCCUGgcCGCGCGu- -3' miRNA: 3'- -CGGUCaCGGUa------UUUGGAC--GCGCGUuu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 124470 | 0.68 | 0.916014 |
Target: 5'- gGCCAGgcaGCCccgcAGCC-GCGCGCAc- -3' miRNA: 3'- -CGGUCa--CGGuau-UUGGaCGCGCGUuu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 93168 | 0.68 | 0.889774 |
Target: 5'- uGCCAGUgguauacaaagGCCAUGAAUCUGCuuaCGCu-- -3' miRNA: 3'- -CGGUCA-----------CGGUAUUUGGACGc--GCGuuu -5' |
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2796 | 5' | -52.9 | NC_001491.2 | + | 137696 | 0.69 | 0.870582 |
Target: 5'- cGgCGGcgGCCGUGGACCgagcgggcgcgggaGCGCGCGAGc -3' miRNA: 3'- -CgGUCa-CGGUAUUUGGa-------------CGCGCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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