Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27963 | 3' | -54.5 | NC_005886.2 | + | 46710 | 0.66 | 0.696243 |
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Target: 5'- -gACGGCcaUCGUC--AGGCaGCGCCa -3' miRNA: 3'- uaUGCCGa-AGCAGaaUUCGgCGCGGc -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 10989 | 0.66 | 0.662734 |
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Target: 5'- -cGCGGUUUUGUCga-GGCCuugcaaGCGCUGu -3' miRNA: 3'- uaUGCCGAAGCAGaauUCGG------CGCGGC- -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 38601 | 0.66 | 0.662734 |
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Target: 5'- -cGuuGCUUgGUgUUGccGGCCGCGCCGc -3' miRNA: 3'- uaUgcCGAAgCAgAAU--UCGGCGCGGC- -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 4792 | 0.67 | 0.617686 |
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Target: 5'- -cACGGUaauuucggUUCGUCacAAGCCgGCGUCGg -3' miRNA: 3'- uaUGCCG--------AAGCAGaaUUCGG-CGCGGC- -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 266 | 0.67 | 0.606431 |
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Target: 5'- cUGCGcaguuaCUUCGUCgau-GCCGCGUCGa -3' miRNA: 3'- uAUGCc-----GAAGCAGaauuCGGCGCGGC- -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 22040 | 0.75 | 0.220977 |
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Target: 5'- -aACGGCUuuUCGUCgucucCCGCGCCGa -3' miRNA: 3'- uaUGCCGA--AGCAGaauucGGCGCGGC- -5' |
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| 27963 | 3' | -54.5 | NC_005886.2 | + | 11740 | 1.07 | 0.001171 |
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Target: 5'- aAUACGGCUUCGUCUUAAGCCGCGCCGa -3' miRNA: 3'- -UAUGCCGAAGCAGAAUUCGGCGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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