Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27964 | 5' | -50.5 | NC_005886.2 | + | 43523 | 0.66 | 0.920901 |
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Target: 5'- cGgUugGGCGggUcauuGGGUUGUucguuucucGUCGUCa -3' miRNA: 3'- aCgGugCUGCuuA----UCCAGCA---------UAGCAG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 18089 | 0.67 | 0.885973 |
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Target: 5'- gGgCugGAUGAcc-GGUCGUGUCGcUCc -3' miRNA: 3'- aCgGugCUGCUuauCCAGCAUAGC-AG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 16806 | 0.67 | 0.885973 |
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Target: 5'- gGCUauaACGACGGcucGGGUCGUA-CGUUc -3' miRNA: 3'- aCGG---UGCUGCUua-UCCAGCAUaGCAG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 24182 | 0.67 | 0.870002 |
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Target: 5'- gGCaACGACGAAcu-GUCGUAUCGa- -3' miRNA: 3'- aCGgUGCUGCUUaucCAGCAUAGCag -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 25136 | 0.67 | 0.852962 |
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Target: 5'- aUGCUucggGCGugauCGGAUcAGGcaUCGUGUCGUCu -3' miRNA: 3'- -ACGG----UGCu---GCUUA-UCC--AGCAUAGCAG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 24274 | 0.69 | 0.765097 |
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Target: 5'- cGCauauGCGACGAAUGGGU-GUgAUCGUg -3' miRNA: 3'- aCGg---UGCUGCUUAUCCAgCA-UAGCAg -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 45999 | 0.69 | 0.765097 |
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Target: 5'- aUGUCGCGACGugcGUAGGU----UCGUCa -3' miRNA: 3'- -ACGGUGCUGCu--UAUCCAgcauAGCAG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 21223 | 0.73 | 0.562683 |
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Target: 5'- cGCUACGcGCGAcaacuAUcAGGUgCGUGUCGUCg -3' miRNA: 3'- aCGGUGC-UGCU-----UA-UCCA-GCAUAGCAG- -5' |
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| 27964 | 5' | -50.5 | NC_005886.2 | + | 12858 | 1.1 | 0.002228 |
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Target: 5'- uUGCCACGACGAAUAGGUCGUAUCGUCu -3' miRNA: 3'- -ACGGUGCUGCUUAUCCAGCAUAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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