miRNA: 3'- cauaaGAAGCC-UGCgGCAuGGCUUCa -5'
miRNA: 3'- caUAAGAAGCcuGC-GGCAUGGCUUCA- -5'
miRNA: 3'- -CAUAAGAAGCCUGCGGCAUGGCUUCA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27966 | 5' | -50.4 | NC_005886.2 | + | 13594 | 0.66 | 0.911476 |
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Target: 5'- ----aCUUCGGuACGgCGU-CCGAAGa -3' miRNA: 3'- cauaaGAAGCC-UGCgGCAuGGCUUCa -5' |
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| 27966 | 5' | -50.4 | NC_005886.2 | + | 41275 | 0.72 | 0.590679 |
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Target: 5'- -gGUUCUUCGu-CGcCCGUGCUGAGGUu -3' miRNA: 3'- caUAAGAAGCcuGC-GGCAUGGCUUCA- -5' |
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| 27966 | 5' | -50.4 | NC_005886.2 | + | 13522 | 1.08 | 0.003144 |
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Target: 5'- uGUAUUCUUCGGACGCCGUACCGAAGUa -3' miRNA: 3'- -CAUAAGAAGCCUGCGGCAUGGCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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