Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27970 | 3' | -54.4 | NC_005886.2 | + | 43519 | 0.66 | 0.716615 |
Target: 5'- ---aGCCcgguugGGCGGGUcaUUGGGUUGUUCGu -3' miRNA: 3'- caugCGG------CCGUCCA--AGCCUAGCAAGU- -5' |
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27970 | 3' | -54.4 | NC_005886.2 | + | 43107 | 0.67 | 0.639135 |
Target: 5'- uUGCGUCGGCGuGaUCGGA-CGUUCu -3' miRNA: 3'- cAUGCGGCCGUcCaAGCCUaGCAAGu -5' |
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27970 | 3' | -54.4 | NC_005886.2 | + | 41875 | 0.69 | 0.539427 |
Target: 5'- ---gGCCGGCGcGUUCGGGUUGaUCGc -3' miRNA: 3'- caugCGGCCGUcCAAGCCUAGCaAGU- -5' |
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27970 | 3' | -54.4 | NC_005886.2 | + | 36588 | 0.75 | 0.267617 |
Target: 5'- --cCGCaagCGGCGGGgaCGGGUCGUUCGa -3' miRNA: 3'- cauGCG---GCCGUCCaaGCCUAGCAAGU- -5' |
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27970 | 3' | -54.4 | NC_005886.2 | + | 16697 | 1.09 | 0.001026 |
Target: 5'- cGUACGCCGGCAGGUUCGGAUCGUUCAg -3' miRNA: 3'- -CAUGCGGCCGUCCAAGCCUAGCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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