Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27970 | 5' | -52.9 | NC_005886.2 | + | 5889 | 0.66 | 0.790746 |
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Target: 5'- cGCGaGCGGCUCG-GCUGUCGUc--- -3' miRNA: 3'- uUGCaUGCUGGGCuCGGCAGCAauau -5' |
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| 27970 | 5' | -52.9 | NC_005886.2 | + | 28511 | 0.67 | 0.727267 |
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Target: 5'- -uUGUACGAUUCGAGCCG-CGa---- -3' miRNA: 3'- uuGCAUGCUGGGCUCGGCaGCaauau -5' |
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| 27970 | 5' | -52.9 | NC_005886.2 | + | 16801 | 0.67 | 0.70516 |
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Target: 5'- uAACG-ACGGCUCGGGUCGUaCGUUcgGu -3' miRNA: 3'- -UUGCaUGCUGGGCUCGGCA-GCAAuaU- -5' |
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| 27970 | 5' | -52.9 | NC_005886.2 | + | 35066 | 0.69 | 0.591963 |
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Target: 5'- aAGCGUGCGcgcCCCGcAGCCGg-GUUGUAc -3' miRNA: 3'- -UUGCAUGCu--GGGC-UCGGCagCAAUAU- -5' |
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| 27970 | 5' | -52.9 | NC_005886.2 | + | 42764 | 0.69 | 0.58067 |
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Target: 5'- uGCGUACGAUCa-AGCCGUCGa---- -3' miRNA: 3'- uUGCAUGCUGGgcUCGGCAGCaauau -5' |
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| 27970 | 5' | -52.9 | NC_005886.2 | + | 16731 | 1.05 | 0.002589 |
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Target: 5'- gAACGUACGACCCGAGCCGUCGUUAUAg -3' miRNA: 3'- -UUGCAUGCUGGGCUCGGCAGCAAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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