Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27972 | 3' | -53.7 | NC_005886.2 | + | 42029 | 0.66 | 0.784952 |
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Target: 5'- -gCCCGCcaUGAuuCCGGCuGUCGucgcgauguuGCAAGCa -3' miRNA: 3'- caGGGUG--ACU--GGCUGuCAGC----------UGUUCG- -5' |
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| 27972 | 3' | -53.7 | NC_005886.2 | + | 16282 | 0.66 | 0.743486 |
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Target: 5'- -aCCUACcgucaGACCGGCGcGUaaccCGGCAGGCg -3' miRNA: 3'- caGGGUGa----CUGGCUGU-CA----GCUGUUCG- -5' |
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| 27972 | 3' | -53.7 | NC_005886.2 | + | 43799 | 0.66 | 0.740291 |
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Target: 5'- uGUUCCGCaagGugCGgugcaauugggaauGCAGcCGGCAGGCa -3' miRNA: 3'- -CAGGGUGa--CugGC--------------UGUCaGCUGUUCG- -5' |
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| 27972 | 3' | -53.7 | NC_005886.2 | + | 34162 | 0.67 | 0.689116 |
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Target: 5'- -gUCCACUGGCCGGCAaGUuccgcCGACGuaccacGCg -3' miRNA: 3'- caGGGUGACUGGCUGU-CA-----GCUGUu-----CG- -5' |
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| 27972 | 3' | -53.7 | NC_005886.2 | + | 40811 | 0.68 | 0.666887 |
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Target: 5'- -gCCCACUGuagcgacaacugGCCGACgAGUucaCGACGAuGCg -3' miRNA: 3'- caGGGUGAC------------UGGCUG-UCA---GCUGUU-CG- -5' |
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| 27972 | 3' | -53.7 | NC_005886.2 | + | 17011 | 1.11 | 0.000881 |
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Target: 5'- cGUCCCACUGACCGACAGUCGACAAGCc -3' miRNA: 3'- -CAGGGUGACUGGCUGUCAGCUGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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