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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27972 | 5' | -52.5 | NC_005886.2 | + | 36324 | 0.66 | 0.787647 |
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Target: 5'- aUCGGCggCACGGGCGGCaCGggcgGuGGu- -3' miRNA: 3'- -AGUCGaaGUGCUUGCCG-GCa---CuCUua -5' |
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| 27972 | 5' | -52.5 | NC_005886.2 | + | 19248 | 0.67 | 0.76684 |
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Target: 5'- cUCAGCaUCAuCGaAGCGGCCGuUGcAGAu- -3' miRNA: 3'- -AGUCGaAGU-GC-UUGCCGGC-AC-UCUua -5' |
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| 27972 | 5' | -52.5 | NC_005886.2 | + | 36954 | 0.67 | 0.745433 |
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Target: 5'- aUCAGCUUCACGuccuuguugucGACGGCCagcuuGAAc -3' miRNA: 3'- -AGUCGAAGUGC-----------UUGCCGGcacu-CUUa -5' |
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| 27972 | 5' | -52.5 | NC_005886.2 | + | 29218 | 0.67 | 0.72353 |
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Target: 5'- gCGGCUUCAuCGAACGGCgCGUu----- -3' miRNA: 3'- aGUCGAAGU-GCUUGCCG-GCAcucuua -5' |
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| 27972 | 5' | -52.5 | NC_005886.2 | + | 35365 | 0.67 | 0.72353 |
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Target: 5'- aCA-CUUC-CGGAUGGCCGgaUGAGAAa -3' miRNA: 3'- aGUcGAAGuGCUUGCCGGC--ACUCUUa -5' |
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| 27972 | 5' | -52.5 | NC_005886.2 | + | 17048 | 1.07 | 0.001927 |
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Target: 5'- gUCAGCUUCACGAACGGCCGUGAGAAUa -3' miRNA: 3'- -AGUCGAAGUGCUUGCCGGCACUCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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