Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27973 | 5' | -55.9 | NC_005886.2 | + | 28876 | 0.66 | 0.643406 |
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Target: 5'- uGGGUUCGCucaguuGAaccgggcgaagcAUGCgGGCAAGUu -3' miRNA: 3'- gCCCAAGCGcu----CU------------UACGgCCGUUCGu -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 1990 | 0.66 | 0.630064 |
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Target: 5'- cCGGGaucgauuucaaCGUGAGAGguguaaCCGGCAGGCGu -3' miRNA: 3'- -GCCCaa---------GCGCUCUUac----GGCCGUUCGU- -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 43808 | 0.66 | 0.610062 |
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Target: 5'- -aGGUgcggUGCaauuGGGAAUgcaGCCGGCAGGCAu -3' miRNA: 3'- gcCCAa---GCG----CUCUUA---CGGCCGUUCGU- -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 29315 | 0.66 | 0.610062 |
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Target: 5'- --cGUUCGCGAcaauAUGCCGGCcgcuGCAa -3' miRNA: 3'- gccCAAGCGCUcu--UACGGCCGuu--CGU- -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 32592 | 0.66 | 0.598973 |
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Target: 5'- aGGGUUUGC-AGcg-GCCGGUGAuGCAu -3' miRNA: 3'- gCCCAAGCGcUCuuaCGGCCGUU-CGU- -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 19398 | 0.69 | 0.460345 |
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Target: 5'- uCGGGcguUUCGCcuucuucgaucGAGccgGCCGGCAAGCc -3' miRNA: 3'- -GCCC---AAGCG-----------CUCuuaCGGCCGUUCGu -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 46891 | 0.69 | 0.450342 |
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Target: 5'- -----gCGCGAcGAGUgcGCCGGCAAGCAu -3' miRNA: 3'- gcccaaGCGCU-CUUA--CGGCCGUUCGU- -5' |
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| 27973 | 5' | -55.9 | NC_005886.2 | + | 17689 | 1.08 | 0.000798 |
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Target: 5'- uCGGGUUCGCGAGAAUGCCGGCAAGCAc -3' miRNA: 3'- -GCCCAAGCGCUCUUACGGCCGUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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