Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27974 | 3' | -53.4 | NC_005886.2 | + | 46621 | 0.67 | 0.741716 |
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Target: 5'- cGCGgGUUGGuCGCaGCGUGUGGCGcUGc -3' miRNA: 3'- -CGCaCAGUCuGCGaCGCGCAUUGC-AC- -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 37380 | 0.68 | 0.687235 |
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Target: 5'- -gGUGUCGucGACGCgugGCGCGgcGCa-- -3' miRNA: 3'- cgCACAGU--CUGCGa--CGCGCauUGcac -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 25085 | 0.68 | 0.664975 |
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Target: 5'- cGCGUGUCAaGCGUUcGUGCaucGCGUGa -3' miRNA: 3'- -CGCACAGUcUGCGA-CGCGcauUGCAC- -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 21843 | 0.7 | 0.520843 |
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Target: 5'- aGCGUGUucaCAGAUGCcGCGCGauGCGa- -3' miRNA: 3'- -CGCACA---GUCUGCGaCGCGCauUGCac -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 27599 | 0.71 | 0.478612 |
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Target: 5'- cCGUGUUuguuuGACGCUGCGCGaauCGUu -3' miRNA: 3'- cGCACAGu----CUGCGACGCGCauuGCAc -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 36873 | 0.73 | 0.39969 |
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Target: 5'- aGCGUGUUAGcGCGUUGCuGCGgcGCGc- -3' miRNA: 3'- -CGCACAGUC-UGCGACG-CGCauUGCac -5' |
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| 27974 | 3' | -53.4 | NC_005886.2 | + | 20399 | 1.1 | 0.001182 |
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Target: 5'- uGCGUGUCAGACGCUGCGCGUAACGUGu -3' miRNA: 3'- -CGCACAGUCUGCGACGCGCAUUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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