Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27975 | 5' | -54.9 | NC_005886.2 | + | 38833 | 0.66 | 0.698622 |
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Target: 5'- gCGGUCGccgAGCGGACGUAUccgGUCc- -3' miRNA: 3'- gGCCAGCuagUCGCCUGCGUA---CAGua -5' |
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| 27975 | 5' | -54.9 | NC_005886.2 | + | 13644 | 0.68 | 0.55173 |
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Target: 5'- gUCGGUCGAUCGGCuGAucacCGCAacgagugcggcggaUGUCGg -3' miRNA: 3'- -GGCCAGCUAGUCGcCU----GCGU--------------ACAGUa -5' |
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| 27975 | 5' | -54.9 | NC_005886.2 | + | 25776 | 0.7 | 0.450342 |
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Target: 5'- aCGGgugaCGAUCAcgcaucGCGGcCGCGUGUCGg -3' miRNA: 3'- gGCCa---GCUAGU------CGCCuGCGUACAGUa -5' |
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| 27975 | 5' | -54.9 | NC_005886.2 | + | 26924 | 0.7 | 0.439479 |
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Target: 5'- gCCGGUCGcgUAGUgccgcccGGGCGCAUGaguUCAa -3' miRNA: 3'- -GGCCAGCuaGUCG-------CCUGCGUAC---AGUa -5' |
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| 27975 | 5' | -54.9 | NC_005886.2 | + | 32176 | 0.73 | 0.324276 |
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Target: 5'- aCGGcUCGcgCAGUGGGCGCGUGauUCGc -3' miRNA: 3'- gGCC-AGCuaGUCGCCUGCGUAC--AGUa -5' |
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| 27975 | 5' | -54.9 | NC_005886.2 | + | 20918 | 1.07 | 0.001334 |
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Target: 5'- gCCGGUCGAUCAGCGGACGCAUGUCAUc -3' miRNA: 3'- -GGCCAGCUAGUCGCCUGCGUACAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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