Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27976 | 3' | -47 | NC_005886.2 | + | 38483 | 0.67 | 0.981656 |
Target: 5'- --cGAGAUUCGCCacCCGguucaccggccgcgcUUGGAUCa -3' miRNA: 3'- gaaCUUUAAGCGGaaGGU---------------AGCCUAGc -5' |
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27976 | 3' | -47 | NC_005886.2 | + | 9296 | 0.68 | 0.957447 |
Target: 5'- ---cGAAUUCGUCgaaUUCAUCGGAUUGc -3' miRNA: 3'- gaacUUUAAGCGGa--AGGUAGCCUAGC- -5' |
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27976 | 3' | -47 | NC_005886.2 | + | 38664 | 0.68 | 0.94827 |
Target: 5'- gUUGAGAaUCaCCUcgUCCGUCGGcgCGu -3' miRNA: 3'- gAACUUUaAGcGGA--AGGUAGCCuaGC- -5' |
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27976 | 3' | -47 | NC_005886.2 | + | 28817 | 0.68 | 0.94827 |
Target: 5'- -aUGAuug-CGCCUaCCgAUUGGAUCGa -3' miRNA: 3'- gaACUuuaaGCGGAaGG-UAGCCUAGC- -5' |
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27976 | 3' | -47 | NC_005886.2 | + | 22933 | 0.95 | 0.056664 |
Target: 5'- uCUUGAAAUUCGCCUUCCAUC-GAUCGu -3' miRNA: 3'- -GAACUUUAAGCGGAAGGUAGcCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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