Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27976 | 5' | -51.5 | NC_005886.2 | + | 7870 | 0.66 | 0.826274 |
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Target: 5'- aGGUggUUgCGAcgGUUGCGCAU-CCGUGa -3' miRNA: 3'- -UCGuuAG-GCUa-CAACGCGUAgGGUAC- -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 29156 | 0.68 | 0.754585 |
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Target: 5'- uGGCAuUUCGGUGaUGCGCGuugaacuuaUCCgCAUGg -3' miRNA: 3'- -UCGUuAGGCUACaACGCGU---------AGG-GUAC- -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 17578 | 0.69 | 0.698995 |
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Target: 5'- uGCAAUCCGAUGgcacGuUGCAaUCCGUGa -3' miRNA: 3'- uCGUUAGGCUACaa--C-GCGUaGGGUAC- -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 13643 | 0.73 | 0.441769 |
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Target: 5'- cGCGAUCUGcUGUUGCGCAgccacaCCCGa- -3' miRNA: 3'- uCGUUAGGCuACAACGCGUa-----GGGUac -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 25115 | 0.74 | 0.411722 |
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Target: 5'- cGGCA--UCGAcg-UGCGCAUCCCAUGc -3' miRNA: 3'- -UCGUuaGGCUacaACGCGUAGGGUAC- -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 31945 | 0.74 | 0.373692 |
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Target: 5'- uGCGAUCCGGUGcgaGCGCAgcaCCCGUc -3' miRNA: 3'- uCGUUAGGCUACaa-CGCGUa--GGGUAc -5' |
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| 27976 | 5' | -51.5 | NC_005886.2 | + | 22969 | 1.09 | 0.001638 |
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Target: 5'- cAGCAAUCCGAUGUUGCGCAUCCCAUGa -3' miRNA: 3'- -UCGUUAGGCUACAACGCGUAGGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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