Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27978 | 5' | -58 | NC_005886.2 | + | 23086 | 0.66 | 0.513702 |
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Target: 5'- ----cGAUUaUGCGCagGCGCACUCGGa -3' miRNA: 3'- caccaCUAA-GCGCGg-CGCGUGAGCCc -5' |
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| 27978 | 5' | -58 | NC_005886.2 | + | 24297 | 0.67 | 0.473055 |
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Target: 5'- -cGGUGAUUCGUggcgaaGCCGgGaagaACaUCGGGc -3' miRNA: 3'- caCCACUAAGCG------CGGCgCg---UG-AGCCC- -5' |
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| 27978 | 5' | -58 | NC_005886.2 | + | 13466 | 0.67 | 0.473055 |
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Target: 5'- aUGGUGGUgCGaagaaaGCCGcCGCGCUCGc- -3' miRNA: 3'- cACCACUAaGCg-----CGGC-GCGUGAGCcc -5' |
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| 27978 | 5' | -58 | NC_005886.2 | + | 4168 | 0.69 | 0.353717 |
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Target: 5'- cGUGGUcGAUUCGUaaaGCCG-GCACgUGGGc -3' miRNA: 3'- -CACCA-CUAAGCG---CGGCgCGUGaGCCC- -5' |
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| 27978 | 5' | -58 | NC_005886.2 | + | 32327 | 0.7 | 0.337343 |
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Target: 5'- --aGUGcAUUCGCGauGCGCAC-CGGGg -3' miRNA: 3'- cacCAC-UAAGCGCggCGCGUGaGCCC- -5' |
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| 27978 | 5' | -58 | NC_005886.2 | + | 24964 | 1.1 | 0.000437 |
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Target: 5'- cGUGGUGAUUCGCGCCGCGCACUCGGGu -3' miRNA: 3'- -CACCACUAAGCGCGGCGCGUGAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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