miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2798 3' -46.5 NC_001491.2 + 61822 0.66 0.999956
Target:  5'- uGACAUGgaaguggGAGUAcuaguUGGGGGgcuugGCGUGGu -3'
miRNA:   3'- uCUGUACa------CUCGUu----AUUCCU-----UGCACC- -5'
2798 3' -46.5 NC_001491.2 + 146139 0.66 0.999922
Target:  5'- aGGACccGUGuccGCGc---GGAGCGUGGa -3'
miRNA:   3'- -UCUGuaCACu--CGUuauuCCUUGCACC- -5'
2798 3' -46.5 NC_001491.2 + 44343 0.66 0.999922
Target:  5'- uGGAagaGUGggagGAGCAGguacGGAAgGUGGa -3'
miRNA:   3'- -UCUg--UACa---CUCGUUauu-CCUUgCACC- -5'
2798 3' -46.5 NC_001491.2 + 142443 0.67 0.999774
Target:  5'- gAGACGgg-GGGU----GGGGGCGUGGa -3'
miRNA:   3'- -UCUGUacaCUCGuuauUCCUUGCACC- -5'
2798 3' -46.5 NC_001491.2 + 129975 0.67 0.999633
Target:  5'- uGGCAgaUGUG-GCAGUGgaagcugagggGGuGGACGUGGa -3'
miRNA:   3'- uCUGU--ACACuCGUUAU-----------UC-CUUGCACC- -5'
2798 3' -46.5 NC_001491.2 + 65096 0.7 0.99458
Target:  5'- gGGGCG-GUGGGCGugucGGGAaaaacACGUGGg -3'
miRNA:   3'- -UCUGUaCACUCGUuau-UCCU-----UGCACC- -5'
2798 3' -46.5 NC_001491.2 + 129330 0.71 0.993685
Target:  5'- aGGAUGUGUGGGUugauGUGGGGGgagaugaagAUGUGGu -3'
miRNA:   3'- -UCUGUACACUCGu---UAUUCCU---------UGCACC- -5'
2798 3' -46.5 NC_001491.2 + 120282 0.71 0.992676
Target:  5'- gGGGCAUGggGGGgGGUGuGG-ACGUGGg -3'
miRNA:   3'- -UCUGUACa-CUCgUUAUuCCuUGCACC- -5'
2798 3' -46.5 NC_001491.2 + 113998 0.72 0.981554
Target:  5'- cGACAUGUGAGgAAcgaAGGGGGCGUu- -3'
miRNA:   3'- uCUGUACACUCgUUa--UUCCUUGCAcc -5'
2798 3' -46.5 NC_001491.2 + 102073 0.74 0.960946
Target:  5'- cGugGUGUGAGCGcgGAGGAaauagccgccACGUauGGa -3'
miRNA:   3'- uCugUACACUCGUuaUUCCU----------UGCA--CC- -5'
2798 3' -46.5 NC_001491.2 + 149263 1.13 0.013568
Target:  5'- cAGACAUGUGAGCAAUAAGGAACGUGGg -3'
miRNA:   3'- -UCUGUACACUCGUUAUUCCUUGCACC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.