Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27989 | 3' | -56.1 | NC_005886.2 | + | 42344 | 0.66 | 0.616773 |
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Target: 5'- gCGCGGUggUCGGcCGaUGCUUGaauaCGAGGUu -3' miRNA: 3'- -GCGUCA--AGCC-GC-GCGAACg---GCUUCAu -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 36000 | 0.66 | 0.572291 |
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Target: 5'- uGUAcGgaUGGCGUGUcaUUGCCGAAGUc -3' miRNA: 3'- gCGU-CaaGCCGCGCG--AACGGCUUCAu -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 44935 | 0.67 | 0.539427 |
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Target: 5'- gCGCAGUcgaUCGGaUGCGCagaUUGCgGAAGc- -3' miRNA: 3'- -GCGUCA---AGCC-GCGCG---AACGgCUUCau -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 31987 | 0.67 | 0.52861 |
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Target: 5'- uGCAGUUCGGCGCa----CCGAuGUAg -3' miRNA: 3'- gCGUCAAGCCGCGcgaacGGCUuCAU- -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 25794 | 0.68 | 0.465636 |
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Target: 5'- uCGCGGccgcgugUCGGCGCGUUUGgUGAaucuGGUGc -3' miRNA: 3'- -GCGUCa------AGCCGCGCGAACgGCU----UCAU- -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 17774 | 0.73 | 0.247463 |
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Target: 5'- gGCGGUaCGGgCGUGCUUGCCGGc--- -3' miRNA: 3'- gCGUCAaGCC-GCGCGAACGGCUucau -5' |
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| 27989 | 3' | -56.1 | NC_005886.2 | + | 37001 | 1.07 | 0.000829 |
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Target: 5'- gCGCAGUUCGGCGCGCUUGCCGAAGUAg -3' miRNA: 3'- -GCGUCAAGCCGCGCGAACGGCUUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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