Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27989 | 5' | -59.5 | NC_005886.2 | + | 43551 | 0.66 | 0.437154 |
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Target: 5'- -uUCUcGUCGUCAUuCCUGCCGCg-- -3' miRNA: 3'- ucAGGaCGGCAGUGcGGACGGCGaag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 3378 | 0.68 | 0.339378 |
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Target: 5'- -aUCgUGCCGguuccugCAcCGCCUGCCGUUa- -3' miRNA: 3'- ucAGgACGGCa------GU-GCGGACGGCGAag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 43977 | 0.7 | 0.24557 |
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Target: 5'- --gCCUGCCGgugcgccCAUGCCUGCCGgUg- -3' miRNA: 3'- ucaGGACGGCa------GUGCGGACGGCgAag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 43941 | 0.7 | 0.24557 |
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Target: 5'- --gCCUGCCGgugcgccCAUGCCUGCCGgUg- -3' miRNA: 3'- ucaGGACGGCa------GUGCGGACGGCgAag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 43905 | 0.71 | 0.204591 |
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Target: 5'- --gCCUGCCGgugcgccCAUGCCUGCCgGCUg- -3' miRNA: 3'- ucaGGACGGCa------GUGCGGACGG-CGAag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 41682 | 0.72 | 0.183934 |
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Target: 5'- uGGUCCUGCgucagcauCGUCAuCGCCccgGCCGCg-- -3' miRNA: 3'- -UCAGGACG--------GCAGU-GCGGa--CGGCGaag -5' |
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| 27989 | 5' | -59.5 | NC_005886.2 | + | 37037 | 1.1 | 0.000247 |
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Target: 5'- gAGUCCUGCCGUCACGCCUGCCGCUUCa -3' miRNA: 3'- -UCAGGACGGCAGUGCGGACGGCGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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