Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2799 | 3' | -53.9 | NC_001491.2 | + | 147399 | 0.71 | 0.760184 |
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Target: 5'- --gGCUCcggccugGGCCGCCGGCUCCAgGggcUCGg -3' miRNA: 3'- ccaCGAG-------UUGGUGGUCGAGGUgU---AGC- -5' |
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| 2799 | 3' | -53.9 | NC_001491.2 | + | 79210 | 0.72 | 0.720685 |
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Target: 5'- gGGUuCUCGucggaugGCCACCAGCacagaaUCCACAUCc -3' miRNA: 3'- -CCAcGAGU-------UGGUGGUCG------AGGUGUAGc -5' |
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| 2799 | 3' | -53.9 | NC_001491.2 | + | 104829 | 0.75 | 0.567199 |
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Target: 5'- uGGaGCUCAgcccauagucuACCGcgguggccauguCCAGCUCCACGUCGu -3' miRNA: 3'- -CCaCGAGU-----------UGGU------------GGUCGAGGUGUAGC- -5' |
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| 2799 | 3' | -53.9 | NC_001491.2 | + | 5408 | 0.78 | 0.387257 |
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Target: 5'- --cGCggCGGCCGCCAGCUCCAUAgCGg -3' miRNA: 3'- ccaCGa-GUUGGUGGUCGAGGUGUaGC- -5' |
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| 2799 | 3' | -53.9 | NC_001491.2 | + | 148924 | 1.1 | 0.0037 |
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Target: 5'- gGGUGCUCAACCACCAGCUCCACAUCGc -3' miRNA: 3'- -CCACGAGUUGGUGGUCGAGGUGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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