Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27992 | 5' | -50.3 | NC_005886.2 | + | 17964 | 0.67 | 0.881118 |
Target: 5'- cCCgGACGgcuuguccUUGCCGuCCUGCACaAGCAc- -3' miRNA: 3'- -GG-CUGC--------AACGGUuGGACGUG-UUGUuc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 36974 | 0.66 | 0.910449 |
Target: 5'- gUCGACG--GCCAGCUUGaACAACGu- -3' miRNA: 3'- -GGCUGCaaCGGUUGGACgUGUUGUuc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 10325 | 0.66 | 0.917047 |
Target: 5'- aCGAUGUcuUCAACuUUGCGCAGCAAc -3' miRNA: 3'- gGCUGCAacGGUUG-GACGUGUUGUUc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 4968 | 0.66 | 0.923346 |
Target: 5'- gCGugGUUGCCuuauuguCUUGCAaAGCAGu -3' miRNA: 3'- gGCugCAACGGuu-----GGACGUgUUGUUc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 41521 | 1.11 | 0.002076 |
Target: 5'- gCCGACGUUGCCAACCUGCACAACAAGu -3' miRNA: 3'- -GGCUGCAACGGUUGGACGUGUUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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