Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27992 | 5' | -50.3 | NC_005886.2 | + | 15094 | 0.7 | 0.747275 |
Target: 5'- aCCGACGaUUGCC-----GCGCAGCAAGc -3' miRNA: 3'- -GGCUGC-AACGGuuggaCGUGUUGUUC- -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 34235 | 0.7 | 0.714197 |
Target: 5'- gUCGGCGgaacUUGCCGGCCaguggaccauUGCACAACu-- -3' miRNA: 3'- -GGCUGC----AACGGUUGG----------ACGUGUUGuuc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 24519 | 0.71 | 0.680326 |
Target: 5'- gCGGCGUcacugGCCGGCgcgcucgcgCUGCACAACAc- -3' miRNA: 3'- gGCUGCAa----CGGUUG---------GACGUGUUGUuc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 606 | 0.72 | 0.611556 |
Target: 5'- gUCGcauACGUUGCCugAACCUGCGCAAg--- -3' miRNA: 3'- -GGC---UGCAACGG--UUGGACGUGUUguuc -5' |
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27992 | 5' | -50.3 | NC_005886.2 | + | 41521 | 1.11 | 0.002076 |
Target: 5'- gCCGACGUUGCCAACCUGCACAACAAGu -3' miRNA: 3'- -GGCUGCAACGGUUGGACGUGUUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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