Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27993 | 3' | -55.4 | NC_005886.2 | + | 36865 | 0.66 | 0.680166 |
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Target: 5'- aGGCGGGuagcGUGUuagCGCGuuGCUgCGGCGc -3' miRNA: 3'- cUUGCUU----CACAa--GCGCggCGA-GCCGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 29228 | 0.66 | 0.64685 |
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Target: 5'- cGAACGgcGcGUUUGCaGCgGC-CGGCAu -3' miRNA: 3'- -CUUGCuuCaCAAGCG-CGgCGaGCCGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 32792 | 0.66 | 0.64685 |
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Target: 5'- -cGCGAcGUGUucggcucucguUCGCGCaCGCaaaCGGCAu -3' miRNA: 3'- cuUGCUuCACA-----------AGCGCG-GCGa--GCCGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 10701 | 0.67 | 0.60228 |
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Target: 5'- uGAAUGGAGUGacUGCGCCGgggaaUCGuGCAg -3' miRNA: 3'- -CUUGCUUCACaaGCGCGGCg----AGC-CGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 24962 | 0.69 | 0.452917 |
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Target: 5'- -cACGuGGUGaUUCGCGCCGCgcacUCGGg- -3' miRNA: 3'- cuUGCuUCAC-AAGCGCGGCG----AGCCgu -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 12506 | 0.69 | 0.452917 |
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Target: 5'- --uCGAAGg--UCGCggucuGCUGCUCGGCAa -3' miRNA: 3'- cuuGCUUCacaAGCG-----CGGCGAGCCGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 31875 | 0.71 | 0.368175 |
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Target: 5'- aAACGGcugauGG-GUUaCGCGCUGCUUGGCAa -3' miRNA: 3'- cUUGCU-----UCaCAA-GCGCGGCGAGCCGU- -5' |
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| 27993 | 3' | -55.4 | NC_005886.2 | + | 41971 | 1.08 | 0.000918 |
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Target: 5'- uGAACGAAGUGUUCGCGCCGCUCGGCAu -3' miRNA: 3'- -CUUGCUUCACAAGCGCGGCGAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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