Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27995 | 3' | -55.9 | NC_005886.2 | + | 31946 | 0.7 | 0.367224 |
Target: 5'- gCGAUCCGGUgcGAGCGCagcacccgucugaaGGUCGAugAUGCAg -3' miRNA: 3'- -GUUGGGCCG--UUCGCG--------------UCAGCU--UGCGU- -5' |
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27995 | 3' | -55.9 | NC_005886.2 | + | 24417 | 0.71 | 0.360253 |
Target: 5'- gAAgCCGGCAAcaaaacccguguuGUGCAGcgCGAGCGCGc -3' miRNA: 3'- gUUgGGCCGUU-------------CGCGUCa-GCUUGCGU- -5' |
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27995 | 3' | -55.9 | NC_005886.2 | + | 16262 | 0.71 | 0.353375 |
Target: 5'- gUAACCCGGCAGGCGguacaauacaaGGGCGCAc -3' miRNA: 3'- -GUUGGGCCGUUCGCgucag------CUUGCGU- -5' |
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27995 | 3' | -55.9 | NC_005886.2 | + | 17794 | 0.71 | 0.352521 |
Target: 5'- aCGGCaagaCGGUAGGCGCAGgcgguaCGGGCGUg -3' miRNA: 3'- -GUUGg---GCCGUUCGCGUCa-----GCUUGCGu -5' |
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27995 | 3' | -55.9 | NC_005886.2 | + | 46795 | 1.08 | 0.000797 |
Target: 5'- gCAACCCGGCAAGCGCAGUCGAACGCAu -3' miRNA: 3'- -GUUGGGCCGUUCGCGUCAGCUUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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