Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27997 | 5' | -63 | NC_005887.1 | + | 40947 | 1.03 | 0.00024 |
Target: 5'- cUUGCCGCUGCCGAGCGGGCCCAUGAUg -3' miRNA: 3'- -AACGGCGACGGCUCGCCCGGGUACUA- -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 41610 | 0.75 | 0.050067 |
Target: 5'- gUGCgGCgGCCG-GCGcGGCCCGUGAg -3' miRNA: 3'- aACGgCGaCGGCuCGC-CCGGGUACUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 14189 | 0.72 | 0.081728 |
Target: 5'- aUUGCacgCGCUGUCGGGCGcGGCCUcgGAc -3' miRNA: 3'- -AACG---GCGACGGCUCGC-CCGGGuaCUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 19123 | 0.7 | 0.12483 |
Target: 5'- -cGCCGCUcgcguGCCGucGGCGuGGCCCuUGAa -3' miRNA: 3'- aaCGGCGA-----CGGC--UCGC-CCGGGuACUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 21818 | 0.69 | 0.143387 |
Target: 5'- -cGgCGCUGCCGGGCGcGCCgaAUGAa -3' miRNA: 3'- aaCgGCGACGGCUCGCcCGGg-UACUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 4585 | 0.69 | 0.131239 |
Target: 5'- aUGCCGCgcaacgacgacgGCaaguuGGGCGGGCUCGUGGa -3' miRNA: 3'- aACGGCGa-----------CGg----CUCGCCCGGGUACUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 16529 | 0.69 | 0.13197 |
Target: 5'- -cGCCGCcggcGCCGAGUGGGCgCGUc-- -3' miRNA: 3'- aaCGGCGa---CGGCUCGCCCGgGUAcua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 24545 | 0.68 | 0.151493 |
Target: 5'- -gGCCGCaccuucgGCCGGGC--GCCCAUGAUc -3' miRNA: 3'- aaCGGCGa------CGGCUCGccCGGGUACUA- -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 31045 | 0.68 | 0.164434 |
Target: 5'- -cGCuCGCUGCCGAGCcGGCCg----- -3' miRNA: 3'- aaCG-GCGACGGCUCGcCCGGguacua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 35061 | 0.67 | 0.188216 |
Target: 5'- -gGUCGCUGCCGucgucGGCGGGCgCGa--- -3' miRNA: 3'- aaCGGCGACGGC-----UCGCCCGgGUacua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 34522 | 0.67 | 0.188216 |
Target: 5'- cUGUCGCUGCgaccaCGGGCuuuuGGGCCCGUa-- -3' miRNA: 3'- aACGGCGACG-----GCUCG----CCCGGGUAcua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 32805 | 0.66 | 0.214973 |
Target: 5'- -cGCCGCUucacggGCaCGAacaGCGGGCCgaCGUGAUg -3' miRNA: 3'- aaCGGCGA------CG-GCU---CGCCCGG--GUACUA- -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 26860 | 0.66 | 0.22656 |
Target: 5'- -aGCCGUUGCCGc-CGGGCaCCGacUGGa -3' miRNA: 3'- aaCGGCGACGGCucGCCCG-GGU--ACUa -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 25535 | 0.66 | 0.238671 |
Target: 5'- -cGUCGCcGCCGAGCGGuGCgCGUu-- -3' miRNA: 3'- aaCGGCGaCGGCUCGCC-CGgGUAcua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 25888 | 0.66 | 0.220702 |
Target: 5'- cUGCCGCgcgGUCGAGCGcauGCCCGc--- -3' miRNA: 3'- aACGGCGa--CGGCUCGCc--CGGGUacua -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 36904 | 0.66 | 0.220702 |
Target: 5'- aUGCgGCaggagUGCC-AGCGGGCgCGUGGUc -3' miRNA: 3'- aACGgCG-----ACGGcUCGCCCGgGUACUA- -5' |
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27997 | 5' | -63 | NC_005887.1 | + | 32318 | 0.66 | 0.209373 |
Target: 5'- aUGCCGCUGuUCGugccAGCGcGGCCgcgCGUGAUg -3' miRNA: 3'- aACGGCGAC-GGC----UCGC-CCGG---GUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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