Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27999 | 3' | -52.6 | NC_005887.1 | + | 11025 | 0.66 | 0.765819 |
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Target: 5'- --gCC-CGGUcGGCACGUGGGACguGa -3' miRNA: 3'- aaaGGaGCCGcUCGUGUAUCUUGguC- -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 29511 | 0.67 | 0.71057 |
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Target: 5'- -cUCgaCGGCGGGCGCGUuguAGAACa-- -3' miRNA: 3'- aaAGgaGCCGCUCGUGUA---UCUUGguc -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 16241 | 0.68 | 0.653174 |
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Target: 5'- --gCCggcCGGCGAGCGCAcGGGcACCGu -3' miRNA: 3'- aaaGGa--GCCGCUCGUGUaUCU-UGGUc -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 33951 | 0.68 | 0.618374 |
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Target: 5'- ---gCUCGGCGAGCACGUcGcGugCGGc -3' miRNA: 3'- aaagGAGCCGCUCGUGUAuC-UugGUC- -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 27029 | 0.74 | 0.303931 |
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Target: 5'- --aCCUCGGCGAGCGCGc--GACCGc -3' miRNA: 3'- aaaGGAGCCGCUCGUGUaucUUGGUc -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 34247 | 0.79 | 0.143234 |
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Target: 5'- --gCCUCGGCGAGCGCGgcGAGCUg- -3' miRNA: 3'- aaaGGAGCCGCUCGUGUauCUUGGuc -5' |
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| 27999 | 3' | -52.6 | NC_005887.1 | + | 40478 | 1.06 | 0.001752 |
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Target: 5'- gUUUCCUCGGCGAGCACAUAGAACCAGu -3' miRNA: 3'- -AAAGGAGCCGCUCGUGUAUCUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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