Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27999 | 5' | -57 | NC_005887.1 | + | 14648 | 0.66 | 0.550774 |
Target: 5'- -gCCGGCGCGgugcugCccGUCGCCGGu-- -3' miRNA: 3'- caGGCCGCGCaa----GcaCAGCGGCUucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 16320 | 0.66 | 0.550774 |
Target: 5'- -gCCGGCcUGUUCGagGUCGCCGc-GAa -3' miRNA: 3'- caGGCCGcGCAAGCa-CAGCGGCuuCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 24777 | 0.67 | 0.529405 |
Target: 5'- -aCgGGCGUGUUCGUcgCGCCGGu-- -3' miRNA: 3'- caGgCCGCGCAAGCAcaGCGGCUucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 14539 | 0.67 | 0.487652 |
Target: 5'- cGUCggCGGCGCGUcgaUCGgcaUGUCGCgCGcGGAa -3' miRNA: 3'- -CAG--GCCGCGCA---AGC---ACAGCG-GCuUCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 34831 | 0.67 | 0.477453 |
Target: 5'- cGUUCaGCGUGacgcucggggUCGUGcCGCCGAGGGu -3' miRNA: 3'- -CAGGcCGCGCa---------AGCACaGCGGCUUCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 15558 | 0.68 | 0.471385 |
Target: 5'- -cUCGGCGCGcgcgaccgcgcucgaUUCGcUGaUCGCCGAAGc -3' miRNA: 3'- caGGCCGCGC---------------AAGC-AC-AGCGGCUUCu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 9662 | 0.68 | 0.447516 |
Target: 5'- aUCCGuGCGCuGUaCGcgGUUGCCGAAGc -3' miRNA: 3'- cAGGC-CGCG-CAaGCa-CAGCGGCUUCu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 4908 | 0.69 | 0.400046 |
Target: 5'- -gCCGGCGCGaggugUUCGUG-CGCCuGAuccAGAu -3' miRNA: 3'- caGGCCGCGC-----AAGCACaGCGG-CU---UCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 36167 | 0.7 | 0.350855 |
Target: 5'- cGUCgCGGCGCGUcacCGUGcCgaccgggaucaccguGCCGAGGAa -3' miRNA: 3'- -CAG-GCCGCGCAa--GCACaG---------------CGGCUUCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 15059 | 0.7 | 0.331129 |
Target: 5'- aUUCGGgGCag--GUGUCGCCGAAGGg -3' miRNA: 3'- cAGGCCgCGcaagCACAGCGGCUUCU- -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 36129 | 0.71 | 0.307637 |
Target: 5'- cGUCCGGCGUGgcucggugaUCGUcUCGCCGGu-- -3' miRNA: 3'- -CAGGCCGCGCa--------AGCAcAGCGGCUucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 24472 | 0.71 | 0.300094 |
Target: 5'- -gUUGGCGCGgccgaUCGUGUCGUCGAu-- -3' miRNA: 3'- caGGCCGCGCa----AGCACAGCGGCUucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 30271 | 0.71 | 0.292695 |
Target: 5'- cGUCCGGCGCGacgaUCGUGccgCGCaCGAc-- -3' miRNA: 3'- -CAGGCCGCGCa---AGCACa--GCG-GCUucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 36554 | 0.71 | 0.28544 |
Target: 5'- uUCCGGCagGCGUUCGUG-CGCCu---- -3' miRNA: 3'- cAGGCCG--CGCAAGCACaGCGGcuucu -5' |
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27999 | 5' | -57 | NC_005887.1 | + | 40512 | 1.08 | 0.000592 |
Target: 5'- aGUCCGGCGCGUUCGUGUCGCCGAAGAu -3' miRNA: 3'- -CAGGCCGCGCAAGCACAGCGGCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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