Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28000 | 3' | -57.2 | NC_005887.1 | + | 29498 | 0.66 | 0.541698 |
Target: 5'- --cGUAGCCGGCggccucgaCGGCGGGcgcgUUGUaGAa -3' miRNA: 3'- cauCAUCGGCCG--------GCCGUCC----AACAgCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 13394 | 0.66 | 0.530917 |
Target: 5'- ----aGGCCGGCgCGGCGGac-GUCGGc -3' miRNA: 3'- caucaUCGGCCG-GCCGUCcaaCAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 34960 | 0.66 | 0.509599 |
Target: 5'- ----cGGCgGGCgUGcGCAGGUUGUUGAu -3' miRNA: 3'- caucaUCGgCCG-GC-CGUCCAACAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 26335 | 0.66 | 0.499075 |
Target: 5'- --cGUGGCCGGCgcUGGCcGGGUgcgCGAa -3' miRNA: 3'- cauCAUCGGCCG--GCCG-UCCAacaGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 23572 | 0.67 | 0.478326 |
Target: 5'- -----cGCCGGCCGGCAG--UG-CGAa -3' miRNA: 3'- caucauCGGCCGGCCGUCcaACaGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 17676 | 0.67 | 0.478326 |
Target: 5'- -gAGgauGCCGGCCgcGGCuGGacaaUGUCGAa -3' miRNA: 3'- caUCau-CGGCCGG--CCGuCCa---ACAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 12152 | 0.67 | 0.478326 |
Target: 5'- cGgcGUGGCUGGCCGGCGaGaaGUgGAa -3' miRNA: 3'- -CauCAUCGGCCGGCCGUcCaaCAgCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 24915 | 0.69 | 0.346909 |
Target: 5'- -cAGcAGCCGGaUCGGCAccGUUGUCGAu -3' miRNA: 3'- caUCaUCGGCC-GGCCGUc-CAACAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 26216 | 0.69 | 0.330367 |
Target: 5'- -cGGUGGuuGGCgGGguGGUUGcgcgCGGc -3' miRNA: 3'- caUCAUCggCCGgCCguCCAACa---GCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 40568 | 0.7 | 0.284273 |
Target: 5'- -gGGUAG-CGGCCGacgcGCAGGUcgagcaUGUCGAg -3' miRNA: 3'- caUCAUCgGCCGGC----CGUCCA------ACAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 38046 | 0.73 | 0.186266 |
Target: 5'- -cAGcUGGCCGGCCucGGCgAGGaUGUCGAc -3' miRNA: 3'- caUC-AUCGGCCGG--CCG-UCCaACAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 31682 | 0.74 | 0.171582 |
Target: 5'- ---uUAGCCGGgCCGGCuGGUUcGUCGGc -3' miRNA: 3'- caucAUCGGCC-GGCCGuCCAA-CAGCU- -5' |
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28000 | 3' | -57.2 | NC_005887.1 | + | 40362 | 1.09 | 0.000457 |
Target: 5'- cGUAGUAGCCGGCCGGCAGGUUGUCGAu -3' miRNA: 3'- -CAUCAUCGGCCGGCCGUCCAACAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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