Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28001 | 5' | -60.6 | NC_005887.1 | + | 31912 | 0.66 | 0.386724 |
Target: 5'- --aUGGGUGCACUGc---GCCGUCGGu -3' miRNA: 3'- cagGCCCACGUGGCcgaaCGGCAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 34394 | 0.66 | 0.360861 |
Target: 5'- -gCCGGGcGCGaggcCCGGCcUGCaaucaGUCGAg -3' miRNA: 3'- caGGCCCaCGU----GGCCGaACGg----CAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 36967 | 0.67 | 0.312845 |
Target: 5'- -aCCGGucGCGCUGGCUUcGCCuUCGAg -3' miRNA: 3'- caGGCCcaCGUGGCCGAA-CGGcAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 28920 | 0.67 | 0.308319 |
Target: 5'- -gCUGagcGGUGCGCCgcucgucgcagcagcGGCUUGCCGcCGAc -3' miRNA: 3'- caGGC---CCACGUGG---------------CCGAACGGCaGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 22119 | 0.67 | 0.297952 |
Target: 5'- -gCCGGGUGCgAUUGGCUcGCUGaUCGc -3' miRNA: 3'- caGGCCCACG-UGGCCGAaCGGC-AGCu -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 29608 | 0.7 | 0.195124 |
Target: 5'- uUCCGGGUGUACUGGUucacgcccauauagcUgaucucGCCGUCGu -3' miRNA: 3'- cAGGCCCACGUGGCCG---------------Aa-----CGGCAGCu -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 29772 | 0.71 | 0.175491 |
Target: 5'- -gCCGGGUcggcggacaccaugcGCauGCCGGCg-GCCGUCGAc -3' miRNA: 3'- caGGCCCA---------------CG--UGGCCGaaCGGCAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 34643 | 0.71 | 0.169045 |
Target: 5'- cGUCgaCGaGGcGCGCCGGCUUGCCGguuucCGGa -3' miRNA: 3'- -CAG--GC-CCaCGUGGCCGAACGGCa----GCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 35286 | 0.71 | 0.160205 |
Target: 5'- -gUCGGuGcgcUGCACCGGaaUGCCGUCGAa -3' miRNA: 3'- caGGCC-C---ACGUGGCCgaACGGCAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 23970 | 0.71 | 0.160205 |
Target: 5'- -aUCGGGUucgcgaGCGCCGGCgcGCCGUCu- -3' miRNA: 3'- caGGCCCA------CGUGGCCGaaCGGCAGcu -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 33103 | 0.71 | 0.158916 |
Target: 5'- aGUCgGGGUcGC-CCGGCUUGCguuccucgccguugCGUCGGu -3' miRNA: 3'- -CAGgCCCA-CGuGGCCGAACG--------------GCAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 39847 | 0.84 | 0.017957 |
Target: 5'- aGUCCGGGUGCacgagcagGCCgGGCUcGCCGUCGAu -3' miRNA: 3'- -CAGGCCCACG--------UGG-CCGAaCGGCAGCU- -5' |
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28001 | 5' | -60.6 | NC_005887.1 | + | 40266 | 1.08 | 0.00027 |
Target: 5'- aGUCCGGGUGCACCGGCUUGCCGUCGAc -3' miRNA: 3'- -CAGGCCCACGUGGCCGAACGGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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